记忆滤泡性辅助T细胞细胞与原因不明复发性流产的关联研究

目的 探讨原因不明复发性流产(unexplained recurrent spontaneous abortion,URSA)的发生与记忆滤泡性辅助T(memory Tfh)细胞表达异常是否存在关联.方法 收集15例染色体正常的URSA为流产组,并以15例正常人工流产患者为正常对照组.分离所有患者外周血单个核细胞及蜕膜组织免疫细胞,用流式细胞术(FCM)检测上述细胞中CD4+CXCR5+memory Tfh以及CD4+CXCR5+PD-1 +memory Tfh细胞水平.结果 流产组蜕膜局部CD4+CXCR5+memory Tfh以及CD4+CXCR5+PD-1 +memory Tfh细胞水平显著高于正常对照组(P＜0.05).流产组外周血CD4+CXCR5+memory Tfh以及CD4+CXCR5+PD-1 +memory Tfh细胞水平与正常对照组无明显差异(P＞0.05).结论 早孕妇女蜕膜局部memory Tfh细胞数量增多可能与URSA发生有关,母-胎界面的免疫异常可能导致URSA的发生.

Relationship between memory Tfh cells and unexplained recurrent spontaneous abortion

Objective To discuss the relationship between memory Tfh cells and unexplained recurrent spontaneous abortion (URSA).Methods The peripheral blood and deciduas of 15 URSA patients were got,and 15 normal patients early pregnancy women were taken as the control.The CD4+CXCR5+memory Tfh cells and CD4+CXCR5+PD1+memory Tfh cells were assessed by flow cytometry.Results The CD4+CXCR5+memory Tfh cells and CD4+CXCR5+PD 1 +memory Tfh cells were both significantly increased in deciduas of the URSA patients compared with the control (P＜0.05).While there were no statistical differences of CD4+CXCR5+memory Tfh cells and CD4+CXCR5+PD1+memory Tfh cells in peripheral blood between the two groups (P＞0.05).Conclusion The upregulation of decidual memory Tfh cells in human early pregnancy led to the failure of the pregnancy,abnormal activation of the immune maternal-fetal interface may cause the occurrence of URSA,which provides a basis for further study.