| COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA |
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| 作者姓名： | Fang Liu Jia-bi Wang Yue-hua Liu Ya-gang Zuo Xiao-hong Man |
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| 作者单位： | [1]Department of Dermatology, Beijing Chaoyang Hospital, Beijing 100020 [2]Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100730 [3]Department of Dermatology, China-Japan Friendship Hospital, Beijing 100029 |
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| 摘 要： | CONDYLOMA acuminata (CA) was the secondmost common sexually transmitted disease in Chi-na1caused by human papilloma virus (HPV)·Polymerase chain reaction (PCR) is the most widely usedtechnique to detect HPVs in CA and in the smear of cer-vix·2Immnofluor…
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| 关 键 词： | 荧光免疫PCR 乳头状瘤病毒 湿疣 病毒感染 |
| 收稿时间： | 2006-03-02 |
COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA |
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| Fang Liu Jia-bi Wang Yue-hua Liu Ya-gang Zuo Xiao-hong Man.COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA[J].Chinese Medical Sciences Journal,2006,21(2):125-127. |
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| Authors: | Fang Liu Jia-bi Wang Yue-hua Liu Ya-gang Zuo Xiao-hong Man |
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| Institution: | Department of Dermatology, Beijing Chaoyang Hospital, Beijing 100020. roseliufang@sohu.com |
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| Abstract: | Objective To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA). Methods HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP). Results The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/60), respectively (P<0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%. Conclusion The sensibility of PCR is superior to that of immunofluorescence. |
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| Keywords: | condyloma acuminata human papilloma virus polymerase chain reaction immunofluorescence |
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