| 红鲫Sox基因的聚合酶链反应扩增 |
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| 引用本文： | 伍爱荣,刘冬娥,陈勇. 红鲫Sox基因的聚合酶链反应扩增[J]. 南华大学学报(医学版), 2007, 35(6): 829-830,839 |
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| 作者姓名： | 伍爱荣 刘冬娥 陈勇 |
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| 作者单位： | 湖南环境生物职业技术学院,湖南,衡阳,421001 |
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| 摘 要： | 目的 克隆雌雄红鲫Sox基因.方法 采用聚合酶链反应以特异扩增人SRY基因HMG-box保守区的一对引物以及扩增雌雄红鲫Sox基因(SRY-box gene).结果 雌雄红鲫个体均扩增出与人SRY基因大小相同的片断,大小约为220 bp.结论 红鲫Sox基因大小约为220 bp,且雌雄个体间无性别特异性.
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| 关 键 词： | 红鲫 Sox基因 PCR SRY基因 红鲫 基因 聚合酶链反应 Variety Gene Amplification Reaction Chain 性别特异性 大小 结果 gene 保守区 特异扩增 方法 克隆 |
| 文章编号： | 1672-7444（2007）06-0829-02 |
| 收稿时间： | 2007-03-06 |
| 修稿时间： | 2007-03-06 |
Polymerase Chain Reaction Amplification of Sox Gene of Carrassius Aurratus Red Variety |
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| WU Ai-rong,LIU Dong-e,CHEN Yong. Polymerase Chain Reaction Amplification of Sox Gene of Carrassius Aurratus Red Variety[J]. Journal of Nanhua University(Medical Edition), 2007, 35(6): 829-830,839 |
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| Authors: | WU Ai-rong LIU Dong-e CHEN Yong |
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| Abstract: | Objective To amplify the Sox gene of carrassius aurrams red variety. Methods A pair of premiers were used, which can amplify the conservation sequences of man HMG - box gene. Results The result of the amplification was that one band with the size, about 220 bp, which was equal to that of human SRY gene, was observed both in female and male Carrassius Aurratus Red variety. Conclusion The Sox gene of carrassius aurrams red variety is not different between male and female, and that is about 220 bp. |
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| Keywords: | carrassius aurratus red variety Sox gene PCR SRY gene |
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