| 弓形虫病基因诊断的动物实验研究 |
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| 引用本文： | 陈玲,侯敏,褚宏亮,姚永伟,刘剑南,章子豪,张耀娟. 弓形虫病基因诊断的动物实验研究[J]. 南京医科大学学报(自然科学版), 2003, 23(5): 437-439 |
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| 作者姓名： | 陈玲 侯敏 褚宏亮 姚永伟 刘剑南 章子豪 张耀娟 |
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| 作者单位： | 南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室，南京医科大学寄生虫学教研室 江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029，江苏 南京 210029 |
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| 摘 要： | 目的：建立敏感、特异、稳定的PCR法，用于弓形虫病诊断。方法：选择与合成引物并确立最佳扩增条件，用PCR法检测实验感染鼠血液中弓形虫DNA，并与ELISA法检测循环抗原(CAg)作比较。结果：该PCR检测弓形虫DNA法特异性强，对其他7种寄生虫DNA均不扩增；敏感性高，可检测到0．2pg DNA；检测急性感染弓形虫小鼠血液最早于感染后第2天出现用性，与ELISA法查CAg比较。PCK检测弓形虫DNA法阳性出现时间早、检出率高。结论：该PCR法可应用于弓形虫感染的早期诊断。
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| 关 键 词： | 弓形虫病 聚合酶链反应 循环抗原 小鼠 |
| 文章编号： | 1007-4368(2003)05-0437-03 |
| 修稿时间： | 2003-03-31 |
The Study of the Genetic Diagnosis of Toxoplasmosis in Experimental Animals |
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| CHEN Ling,HOU Min,CHU Hong-liang,YAO Yong-wei,LIU Jian-nan,ZHANG Zi-hao,ZHANG Yao-juan. The Study of the Genetic Diagnosis of Toxoplasmosis in Experimental Animals[J]. Acta Universitatis Medicinalis Nanjing, 2003, 23(5): 437-439 |
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| Authors: | CHEN Ling HOU Min CHU Hong-liang YAO Yong-wei LIU Jian-nan ZHANG Zi-hao ZHANG Yao-juan |
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| Abstract: | Objective: To establish a sensitive, specific and stable PCR method to diagnose toxoplasmosis. Methods:After selecting and preparing primers and settling the best condition, the DNA of toxoplasma gondii in the blood of infected mice was detected by PCR and compared with the method of ELISA to detect circulating antigen(CAg) in the same mice. Results : The PCR method was very seasirive and specific,which could detect as little as 0.2 pg of DNA of T.gondii and there was no cross reaction with the DNAs of seven other parasites.The DNA could be detected as early as 2 days after the infection, which was much earlier than by ELISA. Conclusion:The PCR method can be applied in the early diagnosis of toxoplas-mosis. |
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| Keywords: | toxoplasmosis polymerase chain reaction circulating antigen mice |
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