DEHP诱导的隐睾鼠胰岛素样因子3基因CpG岛甲基化状态研究

目的 研究胰岛素样因子3(insulin-like factor 3,INSL3)基因在邻苯二甲酸二已酯(DEHP)致小鼠隐睾中DNA甲基化的状态.方法 运用"MethPrimer"软件对INSL3基因进行分析.预测CpG岛,通过甲基化特异性PCR(methylation-specific PCR,MS-PCR)检测INSL3基因CpG岛的甲基化状况,并应用RT-PCR检测INSL3基因在正常组与实验组睾丸组织中的表达情况.结果实验组INSL3基凶第一外显子区存在CpG岛甲基化;实验组INSL3基因mRNA表达显著下降.结论 INSL3基因第一外显子区CpG岛的异常甲基化导致INSL3在隐睾鼠睾丸中表达降低,可能与隐睾的发生密切相关.

Analysis of CpG island methylation of INSL3 gene in cryptorchid mice induced by DEHP

Objective To investigate the role of CpG island methylation of INSL3 gene in cryp-torchid mice induced by DEHP. Methods CpG islands in INSL3 gene were predicted with "MethPrim-er" software. The status of methylation of CpG islands in INSL3 gene was analyzed using methylation-specific PCR(MS-PCR). The levels of INSL3 mRNA in rats of the experimental and control group were detected and compared by RT-PCR methods. Results The methylated CpG islands in the first exon of INSL3 were merely noted in cryptorchid mice, while not in normal mice. The levels of INSL3 mRNA reduced significantly in cryptorchid mice. Conclusions The hypermethylation of CpO islands in the first exon of INSL3 may probably cause low INSL3 expression in cryptorchid mice, which can be closely related with the pathogenesis of cryptorchid.