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Semi-quantitative assay of D antigen site density by flow cytometric analysis
Authors:G. Nicholson  A. Lawrence  F. A. Ala  G. W. G. Bird
Affiliation:West Midlands Regional Blood Transfusion Service, Edgbaston, Birmingham, U.K.
Abstract:Summary. This study describes the comparative measurement of D antigen site density using a fluorescent indirect antiglobulin test (FIAT) read by flow cytometry. The test results confirmed the continuum of D antigen strength from weakest D through to R2R2 and also allowed the majority of weak D samples to be adequately distinguished from D-negative samples.
Keywords:antigen density      flow cytometry.
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