Atrophic gastritis: deficient complex I of the respiratory chain in the mitochondria of corpus mucosal cells |
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Authors: | Marju Gruno Nadezhda Peet Andres Tein Riina Salupere Meeli Sirotkina Julio Valle Ants Peetsalu Enn K Seppet |
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Institution: | (1) Department of Pathophysiology, Centre of Molecular and Clinical Medicine, Faculty of Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, Estonia;(2) Department of Surgery, Faculty of Medicine, University of Tartu, Tartu, Estonia;(3) Department of Internal Medicine, Faculty of Medicine, University of Tartu, Tartu, Estonia;(4) Department of Pathology, Tartu University Hospital, Tartu, Estonia;(5) Department of Gastroenterology, Hospital Virgen de la Salud, Toledo, Spain |
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Abstract: | Background Mitochondrial dysfunction is one of the most characteristic properties of the cancer cell. However, it is not known whether
oxidative energy metabolism has already become altered in conditions of atrophic gastritis, a precancerous state of gastric
disease. The purpose of our study was to comparatively characterize oxidative phosphorylation (OXPHOS) in the atrophic and
nonatrophic gastric corpus mucosa.
Methods Mucosal biopsies were taken from 12 patients with corpus dominant atrophic gastritis and from 12 patients with nonatrophic
mucosa (controls). One part of the tissue samples was permeabilized with saponin for analysis of the function of the respiratory
chain using high-resolution respirometry, and another part was used for histopathological examination. The serum level of
pepsinogen I (S-PGI) was determined with a specific enzyme immunoassay (EIA).
Results Compared to the control group, the maximal capacity of OXPHOS in the atrophy group was almost twofold lower, the respiratory
chain complex I-dependent respiration, normalized to complex II-dependent respiration, was reduced, and respiratory control
by ADP in the presence of succinate was increased in the atrophic corpus mucosa. In the whole cohort of the patients studied,
serum S-PGI level correlated positively with complex I-dependent respiration or complex Idependent to complex II-dependent
respiration ratio.
Conclusions Corpus dominant atrophic gastritis is characterized by decreased respiratory capacity and relative deficiency of the respiratory
complex I of mitochondria in the mucosa, the latter defect probably limiting mitochondrial ATP production and energetic support
of the secretory function of the zymogenic mucosal cells. |
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Keywords: | respiratory chain atrophy gastritis gastric mucosa stomach |
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