| 梅花鹿鹿角托盘蛋白SDS-聚丙烯酰胺凝胶电泳指纹图谱研究 |
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| 引用本文： | 牛放,赵雨,唐任能,黄萍,曲晓波. 梅花鹿鹿角托盘蛋白SDS-聚丙烯酰胺凝胶电泳指纹图谱研究[J]. 中国药业, 2010, 19(10): 21-23 |
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| 作者姓名： | 牛放 赵雨 唐任能 黄萍 曲晓波 |
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| 作者单位： | 1. 长春中医药大学中医药与生物工程研发中心,吉林,长春,130117
2. 吉林农业大学中药材学院,吉林,长春,130118 |
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| 摘 要： | 目的建立梅花鹿鹿角托盘蛋白SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）指纹图谱,为鹿托盘药材的质量评价提供新方法。方法采用SDS-PAGE法得到梅花鹿鹿角托盘蛋白的电泳胶片,通过凝胶电泳图像分析软件生成图谱后,经计算机辅助相似度评价系统进行评价并得到对照指纹图谱。结果梅花鹿鹿角托盘蛋白有6个特征带峰,14批样品指纹图谱相似度较高,方法稳定性、精确度、重现性良好。结论该方法实验简单易行、灵敏度高、专属性强,可用于梅花鹿鹿角托盘药材的质量评价。
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| 关 键 词： | 梅花鹿鹿角托盘蛋白 SDS-聚丙烯酰胺凝胶电泳 指纹图谱 |
Study on SDS-PAGE Fingerprints Chromatogram of Sika Antlerbase Proteins |
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| Niu Fang,Zhao Yu,Tang Renneng,Huang Ping,Qu Xiaobo. Study on SDS-PAGE Fingerprints Chromatogram of Sika Antlerbase Proteins[J]. China Pharmaceuticals, 2010, 19(10): 21-23 |
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| Authors: | Niu Fang Zhao Yu Tang Renneng Huang Ping Qu Xiaobo |
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| Affiliation: | 1. Center for New Medicine Research, Changchun University of TCM, Changchun, Jilin, China 130117; 2. College of Traditional Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin, China 130118) |
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| Abstract: | Objective To establish a fingerprint（FP） analysis and new identification method for controlling the quality of proteins from Sika antlerbase by constructing the SDS-PAGE-FP. Methods SDS-PAGE was used with separate gel thickness 15% and concentrate gel thickness 5%.The standard proteins were reference. By similarity calculation after scanning SDS-PAGE with origin6. 1, the reference SDS - PAGE - FP of Sika antlerbase was attained. Results There were six peaks of Sika antlerbase protein bands in SDS - PAGE. The result was presented well with stability, precision and repeatability. Conclusion SDS- PAGE-FP of Sika antlerbase proteins has been established with the feature of simplicity, high sensitivity and strong specificity. The method can be used to identify and evaluate the quality of Sika antlerbase. |
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| Keywords: | Sika antlerbase proteins SDS - PAGE fingerprints chromatogram |
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