A comparison of the DNA of the "R" and "T" strains of mosquito iridescent virus. |
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Authors: | G W Wagner J D Paschke |
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Institution: | Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA |
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Abstract: | The buoyant density (1.7135 g/cm3), percentage GC (53.9%), and melting temperature (Tm = 76.4°) of the DNA of “regular” mosquito iridescent virus (RMIV) and of “turquoise” mosquito iridescent virus (TMIV) are similar although the molecular weights of the two DNAs are different; 243.3 × 106 and 286.7 × 106, respectively. Analyses show that RMIV contains two identical duplex DNA molecules which are about 15% smaller than the single duplex DNA molecule of TMIV. Reassociation studies show that RMIV and TMIV contain about 17 and 30% repetitious DNA, respectively. Homology studies show the two DNAs to be 100% homologous in their DNA nucleotide sequences. We conclude that the DNA of RMIV and TMIV contain identical sequences and that the portion of the TMIV-DNA molecule accounting for the higher molecular weight is composed of repeated sequences common to both strains. |
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Keywords: | Author to whom reprint requests should be addressed |
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