原发性肝癌细胞核和线粒体DNA微卫星不稳定性研究

目的探讨细胞核和线粒体DNA微卫星不稳在原发肝癌发生中的作用及两者的关系.方法采用限制性片段长度多态性(polymerase chain reaction, single strand conformational polymorphism, PCR-SSCP)方法检测原发性肝癌线粒体DNA微卫星不稳(mitochondrial microsatellite instability, mtMSI);采用PCR方法检测细胞核BAT26微卫星位点不稳定性(nuclear microsatellite instability, nMSI).结果 52份肝癌组织检出mtMSI 11例(21.2%),其中仅1个微卫星位点mtMSI阳性者7份(13.5%),有2个微卫星位点mtMSI阳性者4例(7.7%).有4份于BAT26位点检出nMSI,阳性率为7.7%.肝癌mtMSI发生率在患者同性别、年龄、是否合并乙型肝炎病毒感染和肝硬化、AFP是否阳性组无显著性差异(P>0.05),亦未发现mtMSI与nMSI有显著相关.结论 mtMSI在部分原发性肝癌的发生中起重要作用,肝癌mtMSI与nMSI无关.

Nuclear and mitochondrial microsatellite instability in primary hepatocellular carcinoma

Objective To explore the roles of nuclear microsatellite instability (nMSI) at BAT26 and mitochondrial microsatellite instability (mtMSI) in the genesis and development of primary hepatocellular carcinoma. Methods mtMSI was detected using polymerase chain reaction, single strand conformational polymorphism (PCR SSCP) analysis, and nMSI by PCR method. Results Fifty two cases of primary hepatocellular carcinoma were studied for mtMSI and nMSI. The mtMSI in at least one locus was detected in 11 out of 52 (21 2%) cases. Out of the 11 cases with mtSMI, 7 showed mtMSI at one locus (13 5%) and 4 at two loci (7 7%), but none at more than three loci. The nMSI at BAT26 was detected in only 4 out of 52 (7 7%) cases. mtMSI was found not to be associated with sex, age, HBsAg, cirrhosis of liver, AFP level, and nMSI ( P >0 05). Conclusion mtMSI may be involved in the carcinogenesis of some hepatocellular carcinomas, but mtMSI is not associated with nMSI.