HER2/neu原癌基因胞外区片段DNA克隆及其在小鼠中诱导的抗体应答 *

目的：探讨维甲酸在预防和治疗肿瘤中的作用机制，分离受维甲酸调控的靶基因。方法：采用ＡＰ－ＰＣＲ测序、Ｎｏｒｔｈｅｒｎ杂交及序列２同源分析等方法。结果：首次发现ＡＴＲＡ可上调线粒体基因ＡＴＰ酶第六亚单位表达，这种表达上调开始于Ａ－ＴＲＡ诱导早期并持续整个诱导过程（７ｄ）。Ｎｏｒｔｈｅｒｎ杂交结果证实了ＡＴＲＡ对线粒体基因ＡＴＰ酶第六亚单位的上调作用。夺线粒体基因组的转录调控区Ｄ－ｌｏｏｐ序列进行了初

Induction of Antibody Response by Intramuscular Immunization with HER2/neu Oncogene Extracellular Domain DNA in Mice

Objective: To explore the mechanism of prevention and therapy by retinoic acid in cancer and isolate ATRA-target gene and explore the mechanism of expression regulation of mitochondrial gene. Methods: AP-PCR, hybridization, sequencing etc., were used. Results: ATRA up-regulated the expression of mitochondrion gene ATPase subunit 6, the change lasted in whole period of ATRA treatment (from first day to seventh day). The up-regulation was confirmed by Northen blot analysis. Two possible retinoic acid responsive elements (RAREs) were located in the regulation region of mitochondrion genome. Conclusion: The mitochondrion gene expression by ATRA might be regulated through the interaction of retinoic acid receptor (RAR) with RARE in mitochondrion genome