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| bFGF和BDNF对小鼠神经干细胞体外增殖分化的影响 | |
| 引用本文： | 王占尧,曹磊,姬西团,安凤莲.bFGF和BDNF对小鼠神经干细胞体外增殖分化的影响[J].中华神经外科疾病研究杂志,2010,9(5):435-437. |
| 作者姓名： | 王占尧 曹磊 姬西团 安凤莲 |
| 作者单位： | 1. 西安市中心医院神经外科,陕西,西安,710003 2. 第四军医大学西京医院全军神经外科研究所,陕西,西安,710032 3. 榆林市星元医院神经内科,陕西,榆林,719000 |
| 基金项目： | 陕西省自然科学基金资助项目 |
| 摘 要： | 目的观察碱性成纤维生长因子（bFGF）和脑源性神经生长因子（BDNF）对小鼠源性神经干细胞（NSCs）体外增殖及分化的影响。方法用无血清培养与单克隆技术对小鼠胚胎脑组织进行分离、培养,根据培养液中所加营养因子的不同将传代的NSCs分为bFGF组、BDNF组、bFGF＋BDNF组,观察不同组别对NSCs的定向分化作用。结果与bFGF和BDNF组相比,bFGF＋BDNF组细胞分化为神经元的比例较高（P〈0.05）。结论 bFGF可以提高BDNF诱导小鼠NSCs向神经元分化的比例。 |
| 关 键 词： | 神经干细胞 碱性成纤维生长因子 脑源性神经生长因子 小鼠 |
Regulation of bFGF and BDNF on proliferation and differentiation of mouse NSCs in vitro |
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| WANG Zhanyao,CAO Lei,JI Xituan,AN Fenglian.Regulation of bFGF and BDNF on proliferation and differentiation of mouse NSCs in vitro[J].Chinese Journal of Neurosurgical Disease Research,2010,9(5):435-437. | |
| Authors: | WANG Zhanyao CAO Lei JI Xituan AN Fenglian |
| Institution: | 1Neurosurgery of Xi＇an Central Hospital,Xi＇an 710003; 2Institute of Neurosurgery of PLA,Xijing Hospital,Fourth Military Medical University,Xi＇an 710032; 3Department of Neurology,Yulin Xingyuan Hospital,Yulin 719000,China |
| Abstract: | Objective To investigate the regulation of basic fibroblast growth factor （bFGF） and brain-derived neurotrophic factor （BDNF） on the proliferation and differentiation of mouse neural stem cells （NSCs） in vitro.Methods Serum-free medium and single-cell monoclone technique were used to isolate and incubate NSCs from the cortex of embryonic mouse brain. According to the neurotrophic factors added to the medium,NSCs were divided into group bFGF,group BDNF and group bFGF＋BDNF to observe their effects on the differentiation of NSCs.Results The percentage of neurons differentiated from NSCs in group bFGF＋BDNF were much higher than those in the other two groups （P0.05）. Conclusion bFGF can improve the ability of BDNF in inducing the differentiation of mouse NSCs into neurons. |
| Keywords: | Neural stem cells Basic fibroblast growth factor Brain-derived neurotrophic factor Mouse |
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