Transport and storage ofHelicobacter pylori from gastric mucosal biopsies and clinical isolates |
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Authors: | S. W. Han R. Flamm C. Y. Hachem H. Y. Kim J. E. Clarridge D. G. Evans J. Beyer J. Drnec D. Y. Graham |
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Affiliation: | (1) Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, 2002 Holcombe Blvd., 77030 Houston, Texas, USA;(2) Department of Pathology, Veterans Affairs Medical Center and Baylor College of Medicine, 2002 Holcombe Blvd., 77030 Houston, Texas, USA;(3) Division of Molecular Virology, Veterans Affairs Medical Center and Baylor College of Medicine, 2002 Holcombe Blvd., 77030 Houston, Texas, USA;(4) Abbott Laboratories, Abbott Park, Illinois, USA |
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Abstract: | Various transport and storage conditions for the recovery ofHelicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20 % glycerol in a refrigerator (4°C) for 1 and 2 weeks and in a –20°C laboratory freezer for 4 and 12 weeks. Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20 % glycerol, brucella broth with 20 % glycerol and skim milk with 17 % glycerol at room temperature, 4°C, –20°C and –70°C. Storage at 4°C for 1 and 2 weeks resulted inHelicobacter pylori recovery from 81 % and 19 % of biopsies, respectively. Storage at –20°C yieldedHelicobacter pylori recovery in 100 % and 57 % after 4 and 12 weeks, respectively. At room temperature after 6 h, theHelicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at –70°C than –20°C. |
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