超高效液相色谱法测定紫参胶囊中芍药苷含量 |
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引用本文: | 刘晓闯,高家荣,段贤春,张艳艳,韩燕全,陈浩,萧伟.超高效液相色谱法测定紫参胶囊中芍药苷含量[J].安徽医学,2013,34(3):334-336. |
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作者姓名: | 刘晓闯 高家荣 段贤春 张艳艳 韩燕全 陈浩 萧伟 |
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作者单位: | 230031,合肥,安徽中医学院;230031,合肥,安徽中医学院第一附属医院,国家中医药管理局中药制剂三级实验室;安徽中医学院第一附属医院,国家中医药管理局中药制剂三级实验室,合肥,230031;安徽医学高等专科学校,合肥,230601;中药制药过程新技术国家重点实验室,连云港江苏康缘药业股份有限公司,222001 |
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基金项目: | 安徽省康缘中医药科技创新基金,国家中医药重点学科临床中药学建设项目 |
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摘 要: | 目的建立超高效液相色谱法测定紫参胶囊中芍药苷含量的方法。方法采用超高效液相色谱法,色谱柱为WatersAcquity BEH C1(82.1 mm×50 mm,1.7μm),流动相为乙腈-0.1%磷酸溶液(15∶85),流速0.25 ml/min,检测波长230 nm,柱温30℃。结果芍药苷在14~84μg/ml范围内与峰面积线性关系良好,r=0.9997,平均加样回收率为97.3%,RSD=1.93%(n=6)。结论本法准确、简便、重现性好,可用于紫参胶囊中芍药苷的含量测定。
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关 键 词: | 紫参胶囊 含量测定 芍药苷 超高效液相色谱法 |
Determination of paeoniflorin in Zishen capsules by UPLC |
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Institution: | Liu Xiaochuang,Gao Jiarong,Duan Xianchun,et al Anhui University of Traditional Chinese Medicine,the First Affiliated Hospital of Anhui University of TCM,Hefei 230031,China |
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Abstract: | Objective To establish a method for the determination of paeoniflorin in Zisheng capsules.Methods Waters Acquity UPLC BEH Cl8(2.1 mm×50 mm,1.7 μm)column was adopted.The mobile phase consisted of acetonitrile-phosphoric acid solution(15∶85)at the flow rate of 0.25 ml/min.The detecting wavelength was 230 nm,and the column temperature was 30℃.Results Paeoniflorin was well separated and the liner range of calibration was14~84 μg/ml(r=0.9997).The average recovery was 97.3%,and RSD was 1.93%(n=6).Conclusion The method is accurate,simple and highly reproducible,which could be used for the determination of paeoniflorin in Zisheng capsules. |
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Keywords: | Zishen capsules Determination Paeoniflorin UPLC |
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