“十二井穴”针法对局灶性脑缺血大鼠脑皮质及血清肿瘤坏死因子-α含量的影响

目的:探讨“十二井穴”针法对脑缺血损伤后大鼠肿瘤坏死因子TNF-α影响的时效性。方法:雄性Wistar大鼠96只,随机分为正常组、假手术组各8只,脑缺血模型组、针刺治疗组各40只。后两组又分为1、3、6、12、24 h各时段组,每时间段组各8只。采用光化学诱导法制作一侧大脑局灶性脑缺血模型,施以“十二井穴”针法,在造模后1、3、6、122、4 h不同时间段开始进行针刺治疗,治疗结束后处死大鼠。运用放射免疫分析法测定各组动物脑皮质及血清中TNF-α含量。结果:局灶性脑缺血后1 h,脑皮质及血清TNF-α即明显升高,至24 h仍明显高于正常组,P<0.05;针刺后各组动物脑皮质及血清中TNF-α含量与同时段模型组比较明显降低,P<0.05,0.01。结论:针刺“十二井穴”有降低脑缺血大鼠脑组织及血清TNF-α的作用,这可能是“十二井穴”针法治疗缺血性脑血管疾病特别是早期介入治疗的机理之一。

Effect of Acupuncture of Twelve Jing (Well)-points on Cerebral and Serum TNF-α Contents in Rats with Regional Cerebral Ischemia

Objective: To observe the time-effect of needling twelve Jing(well)-points on TNF-α levels in the serum and cerebral tissue in rats with cerebral ischemia(CI).Methods: A total of 96 male Wistar rats were randomized into normal control(n=8),sham-operation(n=8),CI(n=40) and acupuncture(n=40) groups.CI model was established by adopting photochemical method.The twelve Jing-points(bilateral LU 11,LI 1,PC 9,TE 1,HT 9,SI 1) were pricked with a filiform needle(gauge-30)(1 h),(3 h),(6 h),(12 h) and(24 h) after the establishment of CI model.The rats were killed respectively after six days of treatment for taking blood and brain tissue samples.Serum and cerebral TNF-α contents were assayed with radioimmunoassay according to the instructions of reagent kits.Results: Compared with normal control group,cerebral and serum TNF-α contents in CI group at(1 h),(3 h),(6 h),(12 h) and (24 h) after CI increased significantly(P<0.05);while comparison between acupuncture and CI groups showed that cerebral TNF-α contents of acupuncture group and serum TNF-α levels from(1 h) to(24 h) after CI were significantly lower than those of CI group at the same time courses(P<0.05,0.01). No significant differences were found between normal control and sham-operation groups,and between normal control and acupuncture groups in cerebral and serum TNF-α levels(P>0.05).Conclusion: Acupuncture of twelve Jing-points has a definite effect in reducing CI-induced increase of both cerebral and serum TNF-α contents,which may contribute to its action in improving cerebral ischemia in clinic.