Neo-Sensitab纸片扩散法与NCCL SM27-A微量稀释法测定97株酵母对抗真菌药物敏感性的比较

目的比较Neo-Sensitab纸片扩散法和NCCLS微量稀释法的一致性,评价该商品化方案的稳定性。方法同时采用NCCLSM27-A微量稀释法和Neo-Sensitab纸片扩散法测定临床分离的97株酵母对两性霉素B(AMB)、氟康唑(FCZ)、5-氟胞嘧啶(5-FC)、酮康唑(KCZ)和伊曲康唑(ITC)等五种常用抗真菌药物的敏感性。结果两种方法的药敏结果一致率分别为AMB100%,FCZ94.8%,5-FC93.8%,KCZ75.3%和ITC84.5%。结论纸片扩散法和微量稀释法药敏测试结果一致率较高,以FCZ、AMB和5-FC最佳,该方法检测敏感株的准确率较高,而在区分耐药株和中介株方面有所不足。

Comparison of Neo-Sensitab disk diffusion and NCCLS M27-A microdilution method for susceptibility testing of 97 yeast isolates against antifungal agents

Objective To compare the concordance between Neo-Sensitab disk diffusion method and the Standard NCCLS M27-A microdilution method for susceptibility testing of 97 yeast isolates,and evaluate the reliability of this commercial method.Methods The MICs or inhibition zone diameters of the clinical isolates to five antifungal agents were tested by the two methods.Results The agreement rates of the two methods for AmB,FCZ,5-FC,KCZ and ITC were 100%,94.8%,93.8%,75.3% and 84.5% respectively.Conclusion The two methods for susceptibility testing of the clinical isolates had excellent agreement,especially best fitted to AmB,FCZ and 5-FC.The accordance between Neo-Sensitab disk diffusion method and microdilution method was highly comparable,but that was not convenient to be applied in the decision of resistant strains.