| HSV携带BDNF基因重组体促进大鼠脊髓损伤修复的实验研究 |
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| 作者姓名： | Rong R Dan QQ Liu J Hu LQ Wang TH |
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| 作者单位： | 安徽省立医院老年医学科;四川大学华西医院转化神经科学中心 |
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| 基金项目： | 国家自然科学基金(批准号81070991)资助 |
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| 摘 要： | 目的探讨人单纯疱疹病毒(HSV)携带脑源性神经营养因子(BDNF)基因重组体对脊髓损伤(SCI)的治疗效果。方法构建绿色荧光蛋白(GFP)标记的HSV-BDNF转基因重组体,通过坐骨神经注射使其转运入损伤脊髓运动神经元。用BBB评分观察大鼠后肢运动功能变化,探讨BDNF对损伤脊髓运动神经元的保护作用。结果 GFP标记的HSV-BDNF注入坐骨神经后5d能运输到脊髓腹角运动神经元。BDNF转基因治疗可减轻SCI带来的神经元损害,增加神经元存活数量并阻止胞体萎缩,同时改善大鼠后肢运动行为(P<0.05)。结论 HSV携带BDNF基因是一种有效治疗脊髓损伤的备选方法。
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| 关 键 词： | 大鼠 脊髓损伤 脑源性神经营养因子 转基因治疗 |
Experiment study on the treatment of SCI based on HSV carried BDNF transgene technique |
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| Rong R,Dan QQ,Liu J,Hu LQ,Wang TH.Experiment study on the treatment of SCI based on HSV carried BDNF transgene technique[J].Journal of West China University of Medical Sciences,2012,43(2):262-265. |
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| Authors: | Rong Rong Dan Qi-Qin Liu Jia Hu Li-Qun Wang Ting-Hua |
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| Institution: | Cadre's Ward, Anhui Provincial Hospital, Hefei 230001, China. |
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| Abstract: | Objective To explore the effect of brain derived neurotrophic factor(BDNF) transgenic treatment in rats following spinal cord injury(SCI).Methods BDNF gene was cloned into plasmid then enveloped with human single herpes virus(HSV) to construct HSV carried BDNF transgenic recombinant.BDNF recombinant was injected into sciatic nerve to last label in motorneurous in the caudal cords,then ventral motor neurons were counted and the area of cell body was measured.The BBB scores representing motor function in hindlimbs was also recorded.Results Five days were needed for the GFP-HSV to arrive motorneurons from sciatic nerve.BDNF release could increase the number of motroneurons and inhibit neuronal atrophy in injured spinal cord.BDNF administration also improves motor function in hindlimbs.Conclusion BDNF transgene carried by HSV is a useful strategy for the treatment of SCI,indicating its clinic implication in future treatment. |
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| Keywords: | Rat Spinal cord injury Brain derived neurotrophic factor Transgene therapy |
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