| 不同级别人胶质瘤组织中miR-10b表达变化的研究 |
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| 引用本文： | 孔妍玲,于士柱,孙翠云,王虔,李艳艳,张景敏,安同岭,温艳军.不同级别人胶质瘤组织中miR-10b表达变化的研究[J].中国神经肿瘤杂志,2011(2):98-101. |
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| 作者姓名： | 孔妍玲 于士柱 孙翠云 王虔 李艳艳 张景敏 安同岭 温艳军 |
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| 作者单位： | 天津医科大学总医院,天津市神经病学研究所神经病理室,天津市神经损伤变异与再生重点实验室,教育部中枢神经创伤修复与再生重点实验室,天津300052 |
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| 基金项目： | 国家973计划分项目（2010CB529405）; 国家自然科学基金项目（30770827）; 天津市科技攻关计划重点科技攻关专项项目（06YFSZSF01100）; 天津市科技支撑计划重点项目（07ZCKFSF00800）; 天津市科技创新体系及条件平台建设计划项目（10SYSYJC28800）; 天津市应用基础及前沿技术研究计划（10JCZDJC19400）; 天津市高等学校科技发展基金重点项目（2004ZD06）; 天津市高等学校科技发展基金计划项目（20060202） |
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| 摘 要： | 背景与目的:人miR-10b编码基因位于染色体2q31HOXD8基因之间,有研究显示其特异性地高表达于多种肿瘤中,并与肿瘤的侵袭、迁移和远距离转移有关。本研究对不同级别胶质瘤组织及非肿瘤对照脑组织中miR-10b的表达水平进行检测和比较。方法:采用组织微阵列及锁定寡核苷酸原位杂交技术检测56例不同级别胶质瘤及10例非肿瘤对照脑组织中miR-10b的表达水平。结果:对照脑组织及WHOⅠ~Ⅱ级、Ⅲ级及Ⅳ级胶质瘤组织中miR-10b阳性标记指数(LI%)分别为36.87±20.63、18.86±14.04、12.13±11.56及4.93±6.45,4组间差异均有显著性(P<0.05~0.001),miR-10b LI%与肿瘤的良恶性分级呈显著负相关(rs=-0.61,P<0.001)。结论:miR-10b的表达水平与胶质瘤病理分级呈负相关。
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| 关 键 词： | 胶质瘤 微小RNA 侵袭 迁移 |
Study on the Expression of miR-10b in Human Glioma Tissues of Various Grades |
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| Yan-ling Kong,Shi-zhu Yu,Cui-yun Sun,Qian Wang,Yan-yan Li,Jing-min Zhang,Tong-ling An,Yan-jun Wen.Study on the Expression of miR-10b in Human Glioma Tissues of Various Grades[J].Chinese Journal of Neuro-Oncology,2011(2):98-101. |
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| Authors: | Yan-ling Kong Shi-zhu Yu Cui-yun Sun Qian Wang Yan-yan Li Jing-min Zhang Tong-ling An Yan-jun Wen |
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| Institution: | Department of Neuropathology,Neurologic Institute of Tianjin Medical University General Hospital;Tianjin Key Laboratory of Injuries,Variations and Regeneration of Nervous System;Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System,Ministry of Education,Tianjin 300052,P.R.China |
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| Abstract: | BACKGROUND OBJECTIVE： In human,microRNA-10b（miR-10b） is located between HOXD4 and HOXD8.miR-10b has been found to be expressed in various tumor types and involved in invasion,migration and metastasis.In this study,we detected and compared the expression level of miR-10b in non-tumoral control brain tissues and glioma tissues of various histopathological grades.METHODS： The expression levels of miR-10b in 10 non-tumoral control brain tissues and 56 glioma tissues of 4 pathological grades were detected by tissue microarray and locked oligonucleotide probes in situ hybridization.RESULTS： miR-10b labeling indexes（LI%） in the control brain tissues and WHO grade I~II,grade III and grade IV glioma tissues were 36.87±20.63,18.86±14.04,12.13±11.56 and 4.93±6.45,respectively.Differences between each pair of the four groups are statistically significant（P 0.05）.miR-10b LI% was negatively correlated with the pathological grade of gliomas（rs=-0.61,P 0.001）.CONCLUSON： The expression level of miR-10b is negatively associated with the pathological grade of gliomas. |
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| Keywords: | Glioma microRNA Infiltration Migration |
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