应用均匀设计-高通量筛选技术评价丹参有效单体成分抗氧化及海马神经细胞保护作用的最佳配伍

目的：通过均匀设计-高通量筛选（uniform design—high throughput screening，UD—HTS）技术，对丹参多种有效单体成分进行多因素多水平配伍组合样品抗氧化及海马神经细胞保护作用的筛选。方法：具有生物活性的丹参多种有效单体（脂溶性7种和水溶性7种）。按照均匀设计方案，得到各单体之间不同浓度配比的组合样品，通过抗2，2-二苯基-1-苦肼基自由基（2，2-Di（4-tert-octylphenyl）-1-picrylhydrazyl，DPPH）氧化法和细胞学实验检测各组合样品药物活性。结果：通过初筛和复筛，得到A13、PA两个抗氧化及海马神经细胞保护作用最佳配伍组合样品。结论：均匀设计．高通量筛选技术可快速筛选出药效较好的组合样品，适合多因素多水平配比的大规模药物筛选。

Screening the Optimal Combination Ratio of Active Radix Salviae Miltiorrhizae Ingredients for Their Antioxidant and Hippocampal Neurons Protection Effects Using Uniform Design-High Throughput Screening Technology

Objective： To implement uniform design-high throughput screening （UD-HTS） technology for screening the antioxidant and hippocampal neurons protection activities of radix salviae miltiorrhizae （RSM） ingredients using multifactor and multilevel combination design. Methods： Bioactive ingredients of RSM （7 liposoluble and 7 water-soluble） were combined with different ratios through uniform design. The bioactivity of the combination samples were assessed by anti-DPPH oxidation activity method （screening） and cytological experiment （rescreening）. Results： Two optimal combination samples of the antioxidant and hippocampal neurons protection, A13 and PA, were identified. Conclusion： UD-HTS technology is suitable for multifactor and multilevel screening, which can provide rapid screening of optimal combinations.