川芎嗪抗腹膜间皮细胞损伤的实验研究

目的：观察含川芎嗪腹透液体外对腹膜间皮细胞活力，细胞增殖的影响。方法：分离，培养大鼠腹膜间皮细胞，用细胞计数法，四唑氨蓝（MTT）法，乳酸脱氢酶（LDH）法检测腹膜间皮细胞活力，细胞增殖能力。结果：培养的腹膜间皮细胞暴露于常规使用的4．25％高糖透析液30min后，间皮细胞数量显降低，MTT法示OD值显低于正常对照组，LDH释放增加，提示间皮细胞活力降低，增殖受到显抑制，加入低剂量川芎嗪后，间皮细胞数，MTTOD值较之单纯高糖透析液组均显增高，LDH显降低，但随着剂量的加大，川芎嗪的上述作用减低乃至消失，结论：适当剂量的川芎嗪能对抗常规腹透液引起的间皮细胞抑制和损伤，从而具有保护间皮细胞的作用。

Biocompatibility of Ligustrazine-contained Peritoneal Dialysis:Influence on Peritoneal mesothelial cell

Objective: To study the effects of Ligustrazine-contained peritoneal dialysis on viability and proliferation of mesothelial cells(MC). Methods: Injury to cell membrane(by release of lactate dehydrogenase, LDH) and proliferation(by cell counts and by MTT) were measured after exposure to peritoneal dialysis fluid for 30 min. Results: After exposure to the 4.25% PDS, LDH activity increased significantly. The number of cells and OD value by MTT reduced remarkably. After incubation with ligustrazine-contained peritoneal dialysis fluid, LDH activity decreased significantly. The number of cells and OD value by MTT increased remarkably. Conclusion: ligustrazine-contained peritoneal dialysis fluid has the effect of protecting mesothelial cells from damage by 4.25% peritoneal dialysis fluid.