灵芝菌丝体多糖对K562细胞的抑制作用及免疫调节作用研究

目的研究灵芝菌丝体多糖（ganoderma lucidum mycelia polysaccharides,GLMP）对K562白血病细胞及小鼠体外脾淋巴细胞增殖的影响。方法 MTT法检测不同浓度GLMP对K562白血病细胞增殖及对小鼠体外脾淋巴细胞增殖的作用;血细胞计数方法绘制生长曲线。结果不同浓度的GLMP处理K562细胞48h后,50mg/L及以上浓度抑制率与对照组比较差异有统计学意义（P〈0.01）,而且随浓度的增加抑制率增加。作用48h的IC50为270.2mg/L。生长曲线也表明随时间的延长和浓度的增加,细胞增殖能力下降。GLMP作用小鼠体外脾淋巴细胞48h后,在多糖浓度为50、100mg/L和25、50、100mg/L时能显著刺激静止性和激活型细胞的增殖。结论 GLMP可以抑制K562白血病细胞增殖而且具有免疫调节能力。

The inhibition effects on K562 leukemia cell and immunological regulation effect of Ganoderma lucidum mycelia polysaccharides in vitro

Objective To investigate the effect of Ganoderma lucidum mycelia polysaccharides （GLMP） on K562 leukemia cell and mouse spleen lymphocyte in vitro.Methods MTT assay was used to determine the effect of GLMP on the proliferation of K562 leukemia cell and spleen lymphocyte of mice. The cells number was counted by blood corpuscle counting every day,and the cell growth curve was made according to cell number.Results After K562 cells were treated with different concentrations of GLMP for 48h,the inhibition rates at 50 mg/L and 50mg/L groups were significantly increased,as compared with those of control group （P〈0.01）,furthermore,which was concentration-dependent.The IC50 after treated for 48h was 270.2mg/L.The cell growth curve showed that the cell proliferation ability was also decreased with the time and concentration.After spleen lymphocyte of nice in vitro was treated with GLMP for 48h the GLMP at 50,100mg/L and 25,50,100mg/L could obviously stimulate the proliferation of the cells at stable and active state. Conclusion GLMP can inhibit the proliferation of K562 leukemia cell and is able to regulate immunological function.