锶磷灰石修复下颌骨缺损的实验研究

目的　用不同浓度的锶磷灰石 (Sr-HAP)植入动物体内 ,观察该材料的生物学反应 ,为其临床应用作前期研究。方法　将 2 4只新西兰大白兔分为 3组 ,双侧下颌角均造成 6mm× 12mm× 4mm的缺损 ,用不同浓度 ( 10 % ,5 % ,0 % )的含锶羟磷灰石块分别予以修复 ,术后 1月 ,3月 ,6月时随机处死一组分别进行尸解、四环素荧光标记、定量组织学观察以评估其生物性能。结果　锶磷灰石复合人工骨未引起感染和排斥反应 ,术后材料降解早 ,新生骨大量进入材料间隙 ,其成骨量明显较纯羟基磷灰石为多 ,且统计学上有差异 ,5 %的锶磷灰石烧结体内新生骨成熟度较 10 %者为高 ,但统计学上未显示出成骨量的差异 ,术后 3月时锶磷灰石周边出现强而亮的黄色荧光环 ,术后 6月时荧光环仍未消退。结论　 ( 1)锶磷灰石有良好的组织相容性、骨引导性及生物降解性 ,并具有一定程度的骨诱导性。 ( 2 )锶元素浓度并非是影响总成骨量的关键因素 ,总成骨量可能主要与生物降解的程度大小有关。 ( 3)锶的存在不但提高了新骨的总生成量 ,而且延长了新骨生成的总体时间和高峰期

Experimental Study of Mandibular Reconstruction With Sr-HAP

OBJECTIVE: Different ration of Sr-HAP was implanted in animals to study the bioreactions in order to prepare for the clinical applications in future. METHODS: 24 rabbits were divided into 3 groups for study.Bone defect of 6 x 12 x 4 mm was made at the mandibular angle of rabbits and Sr-HAP of different proportion (10%,5%,0%) was applied to repair the defect. One group of animals were killed at 1 month, 3 months, 6 months to evaluate the biologic capacity with anatomy, tetracycline fluorescent marker and ration histology. RESULTS: Sr-HAP almost hadn't been rejected by hosts, and early after operation the new bone in the Sr-HAP was found with great quantities as the degradation of Sr-HAP much more than that in the pure HA, and the difference of bone quantity can be shown in statistics.New bone in 5% Sr-HAP looked more mature than those in 10% Sr-HAP with no statistic difference in quantity. Strong and bright fluorescent rings can be seen around Sr-HAP from the 3 months to 6 months. CONCLUSION: (1) Sr-HAP has a better biocompatibility, biodegradation than pure HA and an excellent osteoinductivity. (2) The ration of strontium isn't the key influencing the new bone quantity,total new bone quantity maybe associated with the degree of biodegradation. (3) The existence of Sr-HAP improves the total new bone quantity and prolongs the period of new bone increasing.