| HPLC同时测定石崖茶中5种黄酮成分的含量 |
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| 引用本文： | 左文松,徐娟华,周长新,甘礼社. HPLC同时测定石崖茶中5种黄酮成分的含量[J]. 中国中药杂志, 2010, 35(18): 2406-2409 |
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| 作者姓名： | 左文松 徐娟华 周长新 甘礼社 |
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| 作者单位： | 浙江大学,药学院,现代中药研究所,浙江,杭州,310058 |
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| 摘 要： | 目的:建立同时测定民族药石崖茶中5种黄酮成分含量的高效液相色谱方法。方法:采用Chromatorex-C18色谱柱(4.6 mm×250 mm,10μm);流动相0.1%甲酸(A)-甲醇(B),梯度洗脱;检测波长0～15 min,280 nm;检测表儿茶素,15～45 min,262 nm,检测其他4种黄酮类成分,流速1.0 mL.min-1,柱温25℃。结果:山茶苷A、山茶苷B、芹菜素、槲皮苷、表儿茶素分别在1.21～8.06,0.05～0.34,0.10～0.67,0.016～0.105,0.456～3.04μg呈现良好的线性关系,平均加样回收率99.10%,96.81%,100.22%,96.08%,100.30%,RSD分别为2.1%,2.0%,3.0%,3.9%,4.0%。结论:该方法快速、简便、准确,可用于石崖茶的质量控制和评价。
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| 关 键 词： | 石崖茶 黄酮 含量测定 高效液相色谱法 |
| 收稿时间： | 2009-09-15 |
Simultaneous determination of five flavonoid compounds in leaves of Adinandra nitida by HPLC-PAD |
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| ZUO Wensong,XU Juanhu,ZHOU Changxin and GAN Lishe. Simultaneous determination of five flavonoid compounds in leaves of Adinandra nitida by HPLC-PAD[J]. China Journal of Chinese Materia Medica, 2010, 35(18): 2406-2409 |
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| Authors: | ZUO Wensong XU Juanhu ZHOU Changxin GAN Lishe |
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| Affiliation: | Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | |
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| Keywords: | Adinandra nitida camellianin A camellianin B apigenin quercitrin epicatechin HPLC-PAD |
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