异黄酮类药Genistein对原代培养大鼠成骨细胞增殖、分化、钙含量及矿化功能的影响

目的：了解异黄酮类药Genistein对体外培养成骨细胞的作用。方法：应用MTT法、对硝基苯磷酸盐法，原子吸收分光光度法及茜素红染色方法观察Genistein对体外培养成骨细胞的增殖、ALP表达，基质钙含量及矿化结节形成的影响。结果：Genistein具有刺激成骨细胞增殖，提高ALP活性，细胞基质钙含量及矿化结节形成的数量的作用。结论：Genistein具有刺激体外培养成骨细胞增殖，分化成熟及促进矿化的作用。

Effects of Genistein on proliferation,differentiation,content of matrix calcium and mineralization of cultured osteoblasts in vitro

Objective\ To study the effects of Genisteina kind of substance from isoflavont on cultured osteoblasts in vitro. Methods\ MTT,PNPP,atomic absorptiometry and ARS were used to observe the proliferation,activity of ALP,contents of matrix calcium and the number of mineral nodes of osteoblasts cultured in vitro . Results\ It was found that Genistein had the effects on stimulating the proliferation,ALP activity,increasing the contents of matrix calcium and the number of mineral nodes of cultured osteoblasts. Conclusion\ Genistein has the effects on stimulating the proliferation,differentiation,maturation and mineralization of cultured osteoblasts in vitro.