肿瘤坏死因子对正常和Graves病甲状腺细胞生长的调节

研究肿瘤坏死因子（TNF－α）在正常和Graves病（GD）甲状腺生长调节中的作用。方法：采用GD和甲状腺腺瘤旁正常组织，以细胞单层培养及非同位素标记的细胞增殖分析法，检测不同浓度TNF－α对甲状腺细胞生长功能的影响。结果：TNF－α对基础状态下正常甲状腺细胞的生长无明显调节作用，但剂量依赖性地抑制TSH对此类细胞增殖的刺激效应。在10－2～102ng／ml的浓度区间，TNF－α不影响GD甲状腺细胞基础及TSH诱导的生长功能。结论：TNF－α在甲状腺稳态和甲状腺肿大的发病机理中发挥重要作用。

Effect of Tumor Necrosis Factor-alpha on Growth of Human Normal and Hyperfunctioning Thyroid Cells in Monolayer Culture

Objective: To investigate the effect of tumor necrosis factor-alpha (TNF-a) on the growth of normal and Graves' thyroid glands. Methods: Thyroid cells obtained from Graves' disease (GD) and paraadenomatous tissue of thyroid adenomas were cultured in a monolayer system. Non-radioactive proliferation assay were used to detect the growth function of thyrocytes. Results: TNF-a did not affect the proliferation of normal thyrocytes under basal conditions, while it inhibited TSH-induced thyroid cell growth in a dose-dependent manner. However, this cytokine of 10-2~ 102ng/ml exerted no effect on both basal and TSH-stimulated thyrocyte proliferation. Conclusion= TNF-a plays important roles in thyroid haemostasis and in the pathogenesis of goiter.