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Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper |
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| Authors: | Zhang Linbao Gan Junli Ke Changliang Liu Xiaoli Zhao Jianmin You Liping Yu Junbao Wu Huifeng |
| Affiliation: | a Key Laboratory of Coastal Zone Environment Processes, CAS; Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China b Key Laboratory of Fishery Ecology and Environment, Guangdong Province, Guangzhou 510300, PR China c The Graduate School of Chinese Academy of Sciences, Beijing 100049, PR China |
| Abstract: | With the objective to identify promising molecular biomarkers for marine pollution monitoring, a new cytochrome P450 gene was identified from Venerupis (Ruditapes) philippinarum and classified as a member of a new subfamily, CYP414A1. Phylogenetic analysis showed that CYP414A1 was closely related to members of the CYP2 family. CYP414A1 mRNA expression was significantly induced by 50 μg/L B[a]P at 96 h, while no significant change was found in 5 μg/L B[a]P-exposed samples. For heavy metals exposure, the expression of CYP414A1 was significantly up-regulated by Cd but sharply depressed by Cu exposure. These results suggested that CYP414A1 responded to various xenobiotics stresses, and could be used as a candidate biomarker of heavy metals and B[a]P. |
| Keywords: | Venerupis (Ruditapes) philippinarum Cytochromes P450 mRNA expression Benzo[a]pyrene Heavy metals Biomarker |
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