内皮型一氧化氮合酶基因与年龄、吸烟的交互作用

目的 探讨内皮型一氧化氮合酶（eNOS）基因第7外显子G894T（Glu298 Asp）变异与年龄、吸烟之间交互作用在冠心病患病中的意义。方法用病例一病例研究设计，以新诊断的冠心病患者为研究对象，用聚合酶链反应-限制性片段长度多态性分析法（PCR-RFLP）检测eNOS基因G894T变异，运用单因素及多因素Logistic回归分析eNOS基因G894T变异与年龄、吸烟之间的交互作用。结果eNOS基因G894T变异与年龄之间在冠心病的患病中存在显著的相乘模型交互作用，且该交互作用具有显著的剂量-反应关系；eNOS基因G894T变异与吸烟之间在冠心病的患病中无显著的相乘模型交互作用。其交互作用也无显著的剂量-反应关系。结论eNOS基因G894T变异可增加低年龄者冠心病的患病的患病危险性；且年龄越低，eNOS基因G894T变异对冠心病的患病危险性越高。

Interaction between endothelial nitric oxide synthase gene mutation and age, smoking in coronary heart disease

Objective To investigate the effect of interacion between endothelial nitric oxide synthase(eNOS)gene G894T mutation and age,smoking on the risk of coronary heart disease(CHD).Methods Case-only design was used.Newly diagnosed CHD patients were recruited as subjects and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was performed to detect eNOS gene G?894?T mutation.Interaction between eNOS gene G?894?T mutation and age,smoking was evaluated by odds ratios from simple and multiple logistic regression analysis.Results There was significant negative multiplicative model interaction between eNOS gene G?894?T mutation and smoking,nor was the dose-response relationship significant.Conclusion eNOS gene G?894?T mutation can lead to increased risk of CHD in younger groups,and the younger,the higher is the risk.