5-Fu纳米粒涂层人工晶状体抑制兔晶状体后囊膜混浊的实验研究

目的 制备5-Fu纳米粒涂层人工晶状体(IOL)并探讨其对兔晶状体后囊膜混浊的抑制作用的有效性和可行性.方法 通过低能离子束表面氟离子注入技术使5-Fu纳米粒与IOL表面交联黏附形成涂层.取新西兰大白兔40只,随机分为A、B两组,每组20只,对左眼行超声乳化透明晶状体吸出术,对照组为A组,植入普通IOL;实验组为B组,植入5-Fu纳米粒涂层IOL.术后行裂隙灯显微镜、组织病理学及电镜检查.所有数据用SAS统计软件处理,前房闪光和晶状体后囊膜中央视区混浊程度均用Kruskal-Wallis秩和检验进行分析.结果 前房炎性反应:B组的前房闪光轻于A组,差异有统计学意义(x~2=11.245,P=0.024).两组兔眼的前房反应均在术后3 d至1周内缓解.晶状体后囊膜混浊:B组晶状体后囊膜的混浊程度轻于A组,差异有统计学意义(x~2=10.304,P=0.016).光学显微镜行组织病理学检查:A组眼内炎性反应较轻,B组无明显眼内炎性反应表现,A、B两组角巩缘结构及组织均无病理损害.扫描电子显微镜检查:A组见晶状体上皮细胞增生现象,B组未见明显晶状体上皮细胞增生.结论 兔眼透明晶状体超声乳化吸出术后植入5-Fu纳米粒涂层IOL,可有效抑制晶状体后囊膜混浊的发生,眼内毒性较小.

Empirical study on nano-fluorouracil-coated intraocular lens inhibiting posterior capsule opacification

Objective To prepare nano-fluorouracil-coated intraocular lens(IOL) and to investigate its effect and feasibility of inhibiting posterior capsule opacification (PCO) of rabbit.Methods Nano-fluorouracil is cross linked with IOL with the technology of energy-loaded particle beam.Forty New Zealand white rabbits were medially divided into 2 groups named group A and B randomly.All the crystalline lens of the left eyes were emulsified and evacuated of by the same operator, and then different treatment in different groups.In group A, the control group, the Polymethylmethacrylate (PMMA) IOLs were implanted.In group B, the nano-fluorouracil-coated IOLs were implanted.Slit-lamp-micrographscopy,histopathologyscopy and electron-microscopescopy were performed when 4 weeks after the operations.Results The incidence rate and severity degree of PCO in control group is higher than the other group.The inflammatory reaction of anterior segment of the left eyes were remarkably occurred in group A.While almost no inflammatory reaction of anterior segment of the left eyes occurred in group B.Conclusions With implanting nano-fluorouracil-coated IOLs after phacoemulsification and evacuation of the crystalline lens can inhibit PCO actively and have slight toxic reaction and damage in the eyes.