柯萨奇B3病毒抗体与心肌细胞结合的发病作用

目的 研究柯萨奇B3病毒单克隆抗体（CVB3－mAb0与心肌细胞结合，以探讨CVB3－mAb对心肌细胞的致病作用。方法 采用细胞副合技术制备CVB3-mAb，并应用各细胞株分泌的mAb与人、牛、大鼠心肌细胞进行免疫组化分析,比较各株分泌的CVB3－mAb与不同种动物心肌细胞间结合的差异。结果 利用细胞融合技术制备出26种CVB3-mAb。其部分CVB3－mAb可与心肌细胞膜或心肌细胞内的肌原纤维结合，呈棕黄色环状重叠或横纹，CVB3－mAb 2A4，2D4，1G6，1H9，1H10，1H11与人、牛、大鼠心肌细胞均呈阳性反应；CVB3－mAb 2A2仅与人的心肌细胞结合；CVB3－mAb 2B2，2H4，1C10，1G4与人及大鼠心肌细胞结合；CVB3－mAb 2A3与牛和大鼠心肌细胞结合；CVB3－mAb 2G12仅与大鼠心肌细胞结合；CVB3-mAb 1E8仅与牛的心肌细胞结合，且CVB3－mAb 2D4与人心肌的结合为心肌细胞膜，而与牛及大鼠心肌的结合为心肌细胞内的肌原纤维。结论 各CVB3－mAb与不同动物心肌细胞间的结合及结合形式不同；且同一动物的心肌细胞与CVB3－mAb结合有差异。部分柯萨奇B3病毒抗体与动物心肌细胞结合，可引起心肌炎。

Combination of Coxsackievirus B3 monoclonal antibody with cardiac myocytes and its pathopoiesis

AIM To study the combination of Coxsackievirus B 3 monoclonal antibody with cardiac myocytes in order to explore its pathopoiesis to cardiac myocytes. METHODS We prepared Coxsackievirus B 3 monoclonal antibody (CVB 3 mAb) with cell fusion technique, and utilized the CVB 3 mAb secreted by cell strains for immunohistochemical analysis with human, cattle and rat cardiac myocytes, then we compared the difference of combinations between the CVB 3 mAb and different animal cardiac myocytes. RESULTS We prepared 26 strains of Coxsackievirus B 3 monoclonal antibody (CVB 3 mAb). Partial CVB 3 mAb could combine with the membrane or myofibrillae of cardiac myocytes, which showed some light brown ringy overlays or strias. CVB 3 mAb 2A4, 2D4, 1G6, 1H9, 1H10, 1H11 all appeared positive actions with human, cattle and rat cardiac myocytes; CVB 3 mAb 2A2 only combined with human cardiac myocytes; CVB 3 mAb 2B2, 2H4, 1C10, 1G4 with human and rat cardiac myocytes, CVB 3 mAb 2A3 with cattle and rat cardiac myocytes, CVB 3 mAb 2G12 only with rat cardiac myocytes, CVB 3 mAb 1E18 only with cattle and cardiac myocytes, all these combinations existed. CVB 3 mAb 2D4 combined with the membrane of human cardiac myocytes, but combined with the myofibrillae of cattle and rat cardiac myocytes of myofibrillae. CONCLUSION There are differences in the combinations of CVB 3 mAb with cardiac myocytes of different animals or of different CVB 3 mAb with the same animal, and the combined forms are also different. Partial CVB 3 mAb can link with animal cardiac myocytes, which may lead to myocarditis.