单唾液酸神经节苷脂对体外循环后大鼠脑组织Na＋-K＋-ATP酶活性的影响

目的：探讨单唾液酸神经节苷脂（GM1）对大鼠体外循环（CPB）后脑组织的含水量和Na＋-K＋-ATP酶活性的影响。方法：选用雄性SD大鼠，经右颈静脉插管引流，尾动脉插管灌注建立CPB，转流时间1h，建立CPB动物模型。随机分为CPB模型组、CPB模型＋GM。组、输血组和假手术组，每组10只。用干湿法测定脑组织含水量，定磷法测定脑组织的Na＋-K＋-ATP酶活性。结果：CPB24h后，CPB模型组和CPB模型＋GM，组大鼠脑组织含水量均较假手术组明显增加（P〈0．01），但CPB模型＋GM。组大鼠脑组织含水量较CPB模型组明显减少（P〈0．05）；CPB模型组脑组织中的Na＋-K＋-ATP酶活性由正常的（17．03±4．17）mol·Pi／g·protein·h^-1]下降到（12．45±2．64）tool·Pi／g·protein·h。]（Pd0．01），CPB模型＋GM1组下降到（14．72±3．29）mol·Pi／g·protein·h-1]（P〈0．05），与CPB模型组比较差异有统计学意义（P〈0．05）。结论：CPB可抑制Na＋-K＋-ATP酶活性，使脑水肿加重；GM1可起到稳定细胞膜Na＋-K＋-ATP酶的作用。

EFFECT OF MONOSIALOTETERAHEXOSYL GANGLIOSIDE 1 (GM1) ON Na+-K+-ATPase ACTIVITY OF CEREBRAL TISSUE AFTER CARDIOPULMONARY BYPASS IN RATS

Objective：To observe the effect of monosialoteterahexosyl ganglioside I（GM1） on the activity of Na＋-K＋-ATPase of brain tissues after cardiopulmonary bypass（CPB） in rats. Methods： SPF-class adult male SD rats were randomly divided into CPB group, CPB＋ GM1 group, transfusion group and shamoprated group. After establishing the rat model of CPB, GMlwith 20 mg/kg was added to the priming solution. All rats were anesthetized to collect the whole cerebral tissues for detecting the activity of Na＋-K＋- ATPase and water content after 24 hours. Results：The activity of Na＋-K＋-ATPase in cerebral tissues was descended from the normal（17.03±4.17） mol · Pi/g protein · h^-1 to （12.45±2.64） tool · Pi/g protein · h^- 1. GM1 administration obviously enhanced the activity of Na＋-K＋-ATPase to （14.72±3.29） mol · Pi/g protein · h^-1. GM1 can stabilize the functional activity of Na＋-K＋-ATPase of neuronal cytomembrane, and moderate cerebral edema from the brain injuries associated with CPB. Conclusion： CPB inhibits the Na ＋- K＋-ATP activity and increases brain edema. GM1 can stabilize the functional activity of Na＋-K＋-ATPase of neuronal cytomembrane, and moderate cerebral edema from the brain injuries associated with CPB.