油酸聚氧乙烯酯的结构解析表征

目的：对药用辅料油酸聚氧乙烯酯的结构进行解析表征。方法：采用超高效液相-高分辨质谱（UHPLC-HRMS）检测，根据高分辨质谱提供的分子离子峰和碎片信息，获取目标化合物的相对分子质量和结构信息，利用MassHunter质谱工作站及数据分析功能（PCDL、FBF、MFG）对油酸聚氧乙烯酯（460/860）的结构进行分析表征。结果：油酸聚氧乙烯酯主要组分包括聚乙二醇及其单酯和双酯，脂肪酸以油酸为主，同时含有肉豆蔻酸、棕榈酸、棕榈油酸、亚油酸和亚麻酸。油酸聚氧乙烯酯（460）和油酸聚氧乙烯酯（860）两个型号游离聚乙二醇聚合度差别较大。结论：利用UHPLC-HRMS对油酸聚氧乙烯酯的结构进行解析表征，发现其结构中不仅含有药典中所描述的聚乙二醇单酯和双酯，还含有大量未酯化的游离聚乙二醇单体。不同型号的油酸聚氧乙烯酯中聚氧乙烯单酯和双酯的聚合度差别较小，但游离的聚乙二醇差别较大。

Structural Analytical Characterization of Polyoxyl Oleate

Objective:To characterize the structure of pharmaceutical excipients polyoxyl oleate. Methods:UHPLC-HRMS detection was used to obtain the relative molecular mass and the structural information of polyoxyl oleate based on molecular ion peak and fragment information provided by high resolution mass spectrometry. The structure of polyoxyl oleate (460/860) was characterized by Mass Hunter mass spectrometry workstation and data analysis functions (PCDL, FBF, MFG). Results:The main components of polyoxyl oleate included polyethylene glycol, monoesters and diesters. The fatty acids were mainly oleic acid, and contained myristic acid, palmitic acid, palmitoleic acid, linoleic acid and linolenic acid at the same time. Polyoxyl oleate 460 and polyoxyl oleate 860 differed greatly in the degree of polymerization of free polyethylene glycol. Conclusion:UHPLC-HRMS was used to analyze and characterize the structure of polyoxyl oleate. It was found that the structure not only contained polyethylene glycol monoester and diester described in the Pharmacopoeia, but also contained a large number of free polyethylene glycol monomers which were not esterified. The polymerization degree of polyoxyethylene monoester and diester of various types of polyoxyl oleate differed slightly, while the degree of free polyethylene glycol differed greatly.