补正续骨丸对去卵巢大鼠骨量及其相关骨折的作用机制研究

目的探索中药复方补正续骨丸对去卵巢大鼠骨量及相关骨折的影响。方法雌性大鼠卵巢切除法(OVX)造PMOP模型,分组给药6周后DXA行大鼠股骨BMD检测;HE染色测量股骨骨小梁密度;ELISA检测法检测大鼠血清中P1NP、β-CTX;Western blot法检测大鼠股骨中4EBP-1、p70S6K蛋白表达;同时比较不同处理组大鼠骨折愈合时间及相同时间骨痂密度,观察补正续骨丸对OVX大鼠骨折愈合时间及骨痂密度的影响。结果补正续骨丸可增加OVX大鼠股骨BMD、减少骨小梁断裂、降低去卵巢大鼠血清中β-CTX水平、抑制mTOR1信号通路中p70S6K、4EBP1的蛋白表达,补正续骨丸可促进OVX大鼠骨折处骨痂密度增加及减少骨折愈合时间。结论中药复方补正续骨丸通过抑制mTOR1信号通路中p70S6K、4EBP1蛋白的表达抑制骨吸收、促进OVX大鼠骨折的愈合。

Study of the effect of the Buzhengxugu pill on bone mass in ovariectomized rats and the mechanism in ovariectomy-related fractures

Objective To explore the effect of Buzhengxugu pill on bone mass and related fractures in ovariectomized (OVX) rats. Methods Female rats were ovariectomized to establish PMOP model. BMD of femur was detected with DEXA after 6 weeks of drug administration. Trabecular bone density of the femur was measured by HE staining. P1NP and beta-CTX were detected using ELISA assay. Protein expressions of 4EBP-1 and p70S6K in the femur were detected with Western blotting. The effect of Buzhengxugu pill on fracture healing time and callus density of OVX rats was observed. Results Buzhengxugu pill increased BMD of the femur, decreased trabecular fracture, decreased the content of serum beta-CTX in OVX rats, and inhibited the expression of p70S6K and 4EBP1 in mTOR1 signaling pathway. Buzhengxugu pill promoted callus density at the fracture site and reduced fracture healing time of OVX rats. Conclusion Buzhengxugu pill inhibits bone resorption and promotes fracture healing in OVX rats by inhibiting the expression of p70S6K and 4EBP1 protein in mTOR1 signaling pathway.