| 基于HPLC-UV特征图谱与主成分分析的蔓荆子配方颗粒的质量控制研究 |
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| 引用本文： | 张永苗,张兵,梁春霞,彭辉,王仁兴,薛志峰,刘志东.基于HPLC-UV特征图谱与主成分分析的蔓荆子配方颗粒的质量控制研究[J].天津中医药大学学报,2019,36(9):925-931. |
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| 作者姓名： | 张永苗 张兵 梁春霞 彭辉 王仁兴 薛志峰 刘志东 |
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| 作者单位： | 天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617,天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 301617;天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 301617 |
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| 基金项目： | 天津市科技重大专项与工程-新药创制科技重大专项（18ZXXYSY00140）。 |
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| 摘 要： | 目的]通过对蔓荆子配方颗粒中原儿茶酸、对羟基苯甲酸和蔓荆子黄素的含量测定与指纹图谱的研究，结合主成分分析研究不同产地蔓荆子的配方颗粒的质量。方法]采用高效液相色谱-紫外检测器（HPLC-UV）对蔓荆子配方颗粒中原儿茶酸、对羟基苯甲酸和蔓荆子黄素进行含量测定，及其指纹图谱的绘制。色谱柱为Agilent ZOBRAX Plus C18（4.6 mm×250 mm，5 μm），以0.1%磷酸水（A）-乙腈（B）为流动相进行梯度洗脱，流速1.0 mL/min，检测波长为程序波长，柱温35℃。采用SIMCA 14.1软件对指纹图谱特征峰进行主成分分析。结果]10批蔓荆子配方颗粒中的原儿茶酸、对羟基苯甲酸、蔓荆子黄素的含量最大相差分别为2.0倍、2.8倍、8.6倍，其中江西产地蔓荆子的配方颗粒3种活性成分含量均较高。不同产地蔓荆子的配方颗粒相似度在0.914~0.993之间，表明颗粒剂中的化学成分总体稳定。综合含量测定、指纹图谱与主成分分析的结果，可区分出江西产地蔓荆子的配方颗粒质量最佳，均一性良好。结论]基于HPLC-UV法的含量测定结合指纹图谱分析与主成分分析的评价方法，在一定程度上可表征蔓荆子配方颗粒在化学成分方面的共性与差异，可以为蔓荆子配方颗粒甄选原药材、质量控制和评价提供相应的依据。
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| 关 键 词： | 蔓荆子 配方颗粒 原儿茶酸 对羟基苯甲酸 蔓荆子黄素 主成分分析 |
| 收稿时间： | 2019/5/22 0:00:00 |
Quality evaluation of Viticis Fructus formula granules based on HPLC-UV characteristic spectrum and principal component analysis |
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| ZHANG Yongmiao,ZHANG Bing,LIANG Chunxi,PENG Hui,WANG Renxing,XUE Zhifeng and LIU Zhidong.Quality evaluation of Viticis Fructus formula granules based on HPLC-UV characteristic spectrum and principal component analysis[J].Journal of Tianjin University of Traditonal Chinese Medicine,2019,36(9):925-931. |
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| Authors: | ZHANG Yongmiao ZHANG Bing LIANG Chunxi PENG Hui WANG Renxing XUE Zhifeng and LIU Zhidong |
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| Institution: | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China and Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;Tianjin State Key Laboratory of Modern Chinese Medicine-Province and Ministry Co-Established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China |
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| Abstract: | |
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| Keywords: | Viticis Fructus formulated granule parahydroxybenzoic acid protocatechuic acid viticin principal component analysis |
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