内镜超声引导下细针抽吸术获取胰腺癌组织构建体外三维类器官模型的前瞻性临床研究

目的 探讨通过内镜超声引导下细针抽吸术（EUS-FNA）获取胰腺癌组织构建体外三维类器官（organoid）模型的可行性。方法 自2017年6月至2018年1月前瞻性纳入发现胰腺占位并行EUS-FNA诊断的患者9例，术中使用COOK公司22 G穿刺针穿刺获取病灶组织构建体外类器官模型，并持续观察其体外生长状态。结果 9例患者EUS-FNA穿刺的胰腺病灶组织标本，其中5例体外类器官模型构建成功。这5例组织标本经组织病理学诊断均证实为胰腺癌。随着传代次数增加，类器官的生长速度相应加快，对类器官进行HE染色显示类器官与其来源的人胰腺病灶组织病理形态大致相似。结论 通过EUS-FNA穿刺获取人胰腺病灶组织，可以成功构建体外三维类器官模型。胰腺癌类器官模型的建立为临床上胰腺肿瘤患者精准治疗策略的制定提供了绝佳的模型。

Establishment of pancreatic cancer organoids with endoscopic ultrasonography-guided fine-needle aspiration specimen: a prospective pilot study

Objective To study the feasibility of establishing human 3D pancreatic cancer organoids with endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) specimen. Methods A total of 9 patients with suspected pancreatic masses were prospectively included in this study from June 2017 to January 2018. EUS-FNA was performed for initial diagnosis. The biopsy tissues were obtained from a COOK 22-gauge FNA needle for organoid establishment, and the growth status in vitro was observed. Results Nine specimens of pancreatic lesions obtained from EUS-FNA were enrolled. Successful establishment of organoids was achieved in 5 cases, which were all confirmed to be pancreatic cancer histopathologically. In the process of generating, the growth rate of organoids increased correspondingly. The pathological morphology of these organoids was similar to the corresponding pancreatic tissues in HE staining. Conclusion Pancreatic cancer organoids can be successfully created by means of EUS-FNA. Establishment of these organoids can potentially provide excellent models for patients with pancreatic cancer in guiding precision treatment.