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| 肠道真菌菌群失调对小鼠角膜创伤修复的影响 | |
| 引用本文： | 贾路路,路顶立,刘素素,陈雨晴,李志杰,王丽娅.肠道真菌菌群失调对小鼠角膜创伤修复的影响[J].国际眼科杂志,2019,19(5):724-728. |
| 作者姓名： | 贾路路 路顶立 刘素素 陈雨晴 李志杰 王丽娅 |
| 作者单位： | 中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室,中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室,中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室,中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室,中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室; 中国广东省广州市,暨南大学医学院眼科研究所 暨南大学再生医学教育部重点实验室,中国河南省郑州市,郑州大学人民医院 河南省立眼科医院 河南省眼科研究所 河南省人民医院眼科 河南省眼科学与视觉科学重点实验室 |
| 基金项目： | 国家自然科学基金河南联合基金重点支持项目(No.U1704283); 国家自然科学基金面上项目(No.81670827); 郑州市人才队伍建设计划—科技创新团队(No.131PCXTD620); 河南省科技发展计划(No.20150213) |
| 摘 要： | 目的：观察抗真菌药物诱导的肠道真菌菌群失调对小鼠角膜创伤修复的影响。 方法：选用健康无眼疾的C57BL/6J雄性小鼠，将实验小鼠随机分为两组：对照组和两性霉素B组。对照组给予正常饮食，两性霉素B组给予添加两性霉素B的饮食，以诱导小鼠肠道真菌菌群失调，4wk后对两组小鼠角膜进行上皮创伤，使用荧光素钠染色角膜创伤区域，动态观察上皮修复情况，并利用免疫荧光染色观察角膜上皮细胞、炎症细胞变化，通过HE染色观察角膜厚度的变化。 结果：两性霉素B组与对照组相比，再上皮化速度和创伤修复延迟，炎症细胞明显减少，角膜上皮厚度变薄。 结论：肠道真菌菌群失调延迟角膜创伤的修复，降低角膜创伤后的炎症反应。 |
| 关 键 词： | 肠道真菌菌群失调 角膜 创伤修复 炎症反应 |
| 收稿时间： | 2018/11/6 0:00:00 |
| 修稿时间： | 2019/4/3 0:00:00 |
Effect of intestinal fungal dysbiosis on corneal wound healing in mice |
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| Lu-Lu Ji,Ding-Li Lu,Su-Su Liu,Yu-Qing Chen,Zhi-Jie Li and Li-Ya Wang.Effect of intestinal fungal dysbiosis on corneal wound healing in mice[J].International Journal of Ophthalmology,2019,19(5):724-728. | |
| Authors: | Lu-Lu Ji Ding-Li Lu Su-Su Liu Yu-Qing Chen Zhi-Jie Li and Li-Ya Wang |
| Institution: | People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China,People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China,People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China,People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China,People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China; Institute of Ophthalmology, Jinan University Medical School, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China and People''s Hospital of Zhengzhou University, Henan Eye Hospital, Henan Eye Institute, Henan Provincial People''s Hospital, Henan Key Laboratory of Ophthalmology and Vision Science, Zhengzhou 450003, Henan Province, China |
| Abstract: | AIM: To investigate the effect of intestinal fungal dysbiosis induced by antifungal drug on corneal wound healing in mice. METHODS: Male C57BL/6J mice(free of eye disease)were divided into two groups randomly: control(Ctrl)group and amphotericin B treated(Amph)group, The Ctrl group was given a normal diet, and the Amph group was supplemented with amphotericin B to induce intestinal fungal dysbiosis. After 4wk intervention, corneal epithelial trauma was implemented in both groups. Corneal fluorescein staining was used to evaluate the corneal wound area dynamically. Immunofluorescence staining was applied to observe the changes of corneal epithelial cells and inflammatory cells. HE staining was used to assess the change of corneal thickness. RESULTS: Compared with Ctrl group, Amph group had delayed re-epithelialization rate and wound repair, less inflammatory cells and thinner corneal. CONCLUSION: Intestinal fungal dysbiosis delays the corneal wound healing, leading to a weak inflammatory response. |
| Keywords: | intestinal fungal dysbiosis corneal wound healing inflammatory response |
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