| 白藜芦醇抑制TXNIP/NLRP3信号通路改善脂多糖诱导的肾小管上皮细胞炎性损伤 |
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| 引用本文： | 崔勇和,沈先敏.白藜芦醇抑制TXNIP/NLRP3信号通路改善脂多糖诱导的肾小管上皮细胞炎性损伤[J].天津中医药,2019,36(5):495-498. |
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| 作者姓名： | 崔勇和 沈先敏 |
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| 作者单位： | 襄阳市中心医院药学部, 湖北文理学院附属医院, 襄阳 441021,襄阳市中心医院药学部, 湖北文理学院附属医院, 襄阳 441021 |
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| 摘 要： | 目的]观察白藜芦醇对脂多糖诱导的肾小管上皮细胞(HK-2)炎性损伤的保护作用,及其对TXNIP/NLRP3炎性通路的影响。方法]采用脂多糖(LPS,1 mg/L)体外诱导HK-2细胞炎性损伤模型,将细胞分为正常对照组、LPS诱导组、LPS+低剂量(50μmol/L)白藜芦醇组、LPS+中剂量(100μmol/L)白藜芦醇组、LPS+高剂量(200μmol/L)白藜芦醇组;CCK8法检测HK-2细胞相对存活率,酶联免疫吸附测定(ELISA)法检测炎性因子白介素-6(IL-6)、白介素-1β(IL-1β)及肿瘤坏死因子-α(TNF-α)水平,Western Blot方法分析诱导型一氧化氮合酶(iNOS)、环氧酶-2(COX-2)、人硫氧还蛋白互作蛋白(TXNIP)及人隐热蛋白(NLRP3)表达。结果]与LPS诱导组比较,不同浓度白藜芦醇干预的HK-2细胞相对存活率明显升高(P0.05),细胞上清炎性因子IL-6、IL-1β及TNF-α水平明显降低(P0.05),细胞中炎性蛋白iNOS及COX-2表达水平显著降低(P0.05);细胞中TXNIP及NLRP3蛋白表达也显著降低(P0.05),并表现出剂量依赖性。结论]白藜芦醇可以明显抑制LPS诱导的HK-2细胞炎症损伤,抑制炎症相关蛋白的表达,其作用机制可能与抑制TXNIP/NLRP3炎性通路活化相关。
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| 关 键 词： | 白藜芦醇 肾小管上皮细胞 炎性反应 TXNIP/NLRP3信号通路 |
| 收稿时间： | 2018/12/29 0:00:00 |
Effects of resveratrol against inflammatory injury of human renal proximal tubular cells induced by LPS through the inhibition of TXNIP/NLRP3 signaling pathway |
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| CUI Yonghe and SHEN Xianmin.Effects of resveratrol against inflammatory injury of human renal proximal tubular cells induced by LPS through the inhibition of TXNIP/NLRP3 signaling pathway[J].Tianjin Journal of Traditional Chin Medicine,2019,36(5):495-498. |
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| Authors: | CUI Yonghe and SHEN Xianmin |
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| Institution: | Pharmacology Department, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China and Pharmacology Department, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China |
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| Abstract: | |
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| Keywords: | resveratrol human renal proximal tubular cells inflammatory response TXNIP/NLRP3 signaling pathway |
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