不同工艺的绞股蓝总甙降血脂作用的对比研究

目的：建立大鼠高脂血症模型，研究两种工艺的绞股蓝总甙降血脂作用的药效学对比，为绞股蓝总甙的工艺优化奠定基础。方法：90只健康大鼠，随机分为正常对照组、模型组、工艺1低、中、高剂量组、工艺2低、中、高剂量组和阿托伐他汀钙组。除正常对照组外，其余各组均饲喂高脂高胆固醇饲料。连续给药33天后，观察状态、体重，测定血清总胆固醇（Total Cholesterol，TC）、血清甘油三酯（Triglyceride，TG）、血清低密度脂蛋白胆固醇（Low Density Lipoprotein-C，LDL-C）、血清高密度脂蛋白胆固醇（High Density Lipoprotein-C，HDLC）、血清游离脂肪酸（Free Fatty Acids，FFA）、肝组织超氧化物歧化酶（Superoxide Dismutase，SOD）、肝组织还原型谷胱甘肽（Exogenous Reduced Glutathione，GSH）、肝组织丙二醛（Malondialdehydle，MDA）、血清谷丙转氨酶（Alanine Transaminase，ALT）和谷草转氨酶（Aspartate Transminase，AST）。结果：两组工艺的绞股蓝总甙高剂量连续给药可降低模型大鼠血清TC、FFA、LDL-C水平和肝脏MDA含量，升高肝脏还原型GSH的水平和SOD的水平，但工艺2中剂量也可降低模型大鼠血清LDL-C水平，升高肝脏还原型GSH的水平和SOD的水平，这说明工艺2的药效要略优于工艺1。结论：不同工艺的绞股蓝总甙均有降低血脂的作用，但不同工艺的绞股蓝总甙所产生的药效却不尽相同，这为绞股蓝总甙的工艺优化提供科学基础。

Comparative Study on the Hypolipidemic Effect of Gynoglycosides with Different Process

Objective: To establish the rat model of hyperlipidemia, to compare the effects of lowering blood lipids of gynoglycosides which were prepared by two different processes, to provide the evidence for the process optimization of thegynoglycosides from Gynostemma pentaphyllum (Thunb.) Makino. Methods: A total of 90 healthy rats were randomlydivided into 9 groups including the model group, the normal control group, and Process 1 low, Process 1 medium, Process1 high dose group, and Process 2 low, Process 2 medium, Process 2 high dose group, and the atorvastatin calcium group.All groups were fed with high fat and high cholesterol diet except the normal control group. After successive medicationfor 33 days, the status and the weight were observed, and the serum total cholesterol (TC), serum triglyceride (TG), serumLDL cholesterol (LDL-C), serum HDL cholesterol (HDL-C), serum free fatty acids (FFA), superoxide dismutase inhepatic tissue (SOD), reduced glutathione in hepatic tissue (GSH), malondialdehyde in hepatic tissue (MDA), serumalanine aminotransferase (ALT) and serum glutamate transaminase (AST) were measured. Results: Process 1 and Process2 high dose could reduce the TC, FFA, LDL-C and MDA, and increase GSH and SOD. Also, Process 2 medium dosecould reduce the LDL-C, increase GSH and SOD, indicating that the effects of Process 2 was slightly better than Process1. Conclusion: The gynoglycosides of two different processes both can decrease blood lipids, but the effects ofgynoglycosides of these different processes were different. This study provides a scientific basis for the optimization of theprocess of the gynoglycosides.