蛭龙活血通瘀胶囊对心肌梗死大鼠内皮祖细胞动员

目的：探讨蛭龙活血通瘀胶囊对急性心肌梗死（AMI）大鼠内皮祖细胞（endothelial progenitor cells,EPCs）动员。方法：结扎SD大鼠左冠状动脉前降支制作AMI模型,将32只大鼠随机分为4组：AMI组、蛭龙活血通瘀胶囊低剂量组1.56 g/kg/d（简称：低剂量组）、蛭龙活血通瘀胶囊高剂量组6.24 g/kg/d（简称：高剂量组）、和假手术组,术后24 h用药灌胃,1次/d,5周后计数外周血EPCs数量、免疫组化法测定血清血管内皮生长因子（VEGF）的数量。结果：术后5周与假手术组比较,AMI组、低剂量组和高剂量组的血清VEGF数量、外周循环EPCs数量均明显升高（P〈0.05）;术后5周与AMI组比较,低剂量组和高剂量组血清VEGF数量明显增加（P〈0.05）。结论：AMI大鼠5周后骨髓EPCs的动员增加,蛭龙活血通瘀胶囊能促进AMI大鼠骨髓EPCs的动员,增加循环EPCs数量,从而为临床治疗心肌梗死提供新的治疗措施。

Effect of Zhilong Huoxue Tongyu capsule on endothelial progenitor cell mobilization in rats with myocardial infarction

Objective： To investigate the effect of Zhilong Huoxue Tongyu capsule on bilization of endothelial progenitor cells（EPCs） in rats with acute myocardial infarction（AMI）.Methods： Myocardial infarction（MI）was induced by ligation of left coronary artery in SD rats.After 24 h,24 survived rats were randomized into AMI group,Zhilong Huoxue Tongyu capsule low dose group（1.56 g/kg/d） and high dose group（6.24 g/kg/d）.8 rats were operated as sham group.5 weeks after MI, the number of circulating EPCs was counted, Serum VEGF levels were determined by ELISA. Results： Compared with sham group,serum VEGF levels and circulating EPCs were significantly increased in AMI group, high and low dose groups.Compared with the AMI group,there was no difference in serum VEGF level in low and high dose groups,but the number of circulating EPCs increased significantly. Conclusion： Zhilong Huoxue Tongyu capsule can promote the mobilization of marrow EPCs,increasing the number of circulating EPCs.