津力达颗粒对糖尿病大鼠胰岛B细胞的保护作用

目的 探讨津力达颗粒对糖尿病大鼠胰岛B细胞的保护作用。方法 将100只SPF级雄性SD大鼠除对照组外采用高脂饮食,链脲佐菌素腹腔一次性注射诱发糖尿病大鼠模型后随机分为模型组、（低中高剂量）津力达药物组、a-硫辛酸组、胰岛素组、二甲双胍组。各组给予灌胃给药（胰岛素组腹腔注射）。给药2个月后测定体重、空腹血糖（FBG）、胰岛素（FINS）、糖化血红蛋白（HbA1c）、氧化应激及炎症反应指标,并取胰尾组织行胰岛素免疫组化染色观察。结果 与模型组相比,其他各组FBG、HbA1c等均降低;津力达高剂量组SOD、GSH活性明显升高（P<0.01）,IL-1β、MDA含量降低（P<0.01）,胰岛B细胞免疫阳性染色的面积增加（P<0.01）,与糖尿病模型组比较差异有显著意义。结论 津力达颗粒对受损的糖尿病大鼠胰岛B细胞具有保护作用。

The protective effect of Jin lida on islet B cells in diabetes mellitus rats

Objective To investigate the protective effect of Jinlida granules on islet β cells in diabetes mellitus rats.Methods Diabetic models were induced by high-fat diet and intraperitoneal injection of streptozotocin(STZ) in SD rats.The study was divided into model group,Jinlida granule group(0.75,1.5,3.0 g/kg),α-lipoic acid group,insulin group,and metformin group.Each group was given corresponding drugs,with insulin given by subcutaneous injection and other drugs given by intragastric administration.The body weight,fasting blood glucose(FBG),fasting insulin(FINS),glycosylated hemoglobin(HbA1c),the activities of superoxide dismutase(SOD) and glutathione(GSH),and the contents of malondialdehyde(MDA),IL-1β and TNF-α were examined 2 months later.The pancreatic tissues were immunostained for insulin.Results Compared with the model group,the levels of FBG and HbA1c were decreased in other groups(P<0.05,P<0.01).The activities of SOD and GSH were significantly increased(P<0.01) and the levels of MDA,IL-1β and TNF-α were significantly decreased(P<0.01) in high-dose(3.0 g/kg) Jinlida granule group,with increased islet area as shown by pathological staining.Conclusion Jinlida granule has protective effect against STZ-induced islet β cell injury in diabetic rats.