| SELEX技术的靶标范围拓展 |
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| 引用本文： | 徐发良,肖觉.SELEX技术的靶标范围拓展[J].第二军医大学学报,2012,33(4):432-435. |
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| 作者姓名： | 徐发良 肖觉 |
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| 作者单位： | 1. 重庆市肿瘤医院、重庆市肿瘤研究所乳腺疾病治疗中心,重庆,400030
2. 重庆市肿瘤医院、重庆市肿瘤研究所病理科,重庆,400030 |
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| 基金项目： | 重庆市科委科技攻关计划项目(CSTC,2010AC5132)和重庆市卫生局医学科研项目(2010-1-55, 2009-2-122). |
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| 摘 要： | 靶标范围的不断拓展是SELEX技术和其他随机文库技术(如噬菌体表面展示技术等)的显著特征之一。经过20多年发展，SELEX的靶标已经拓展到包括金属离子、各种有机小分子、药物、蛋白质等生物大分子、致病微生物、活细胞和组织等在内的多种形式。以细胞和组织为靶标的SELEX筛选策略在肿瘤靶向诊断和治疗的研究中得到初步应用。
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| 关 键 词： | SELEX 随机寡核酸文库 靶标 适配子 |
| 收稿时间： | 7/7/2011 8:23:48 PM |
| 修稿时间： | 2012/3/12 0:00:00 |
Expanding targets for systematic evolution of ligands by exponential enrichment (SELEX) |
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| XU Fa-liang and XIAO Jue.Expanding targets for systematic evolution of ligands by exponential enrichment (SELEX)[J].Academic Journal of Second Military Medical University,2012,33(4):432-435. |
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| Authors: | XU Fa-liang and XIAO Jue |
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| Institution: | 1. Treatment Centre of Breast Diseases, Chongqing Cancer Hospital and Institute, Chongqing400030, China2. Department of Pathology, Chongqing Cancer Hospital and Institute, Chongqing 400030, China*Corresponding author. |
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| Abstract: | The continuous expanding of targets is a distinct character of systematic evolution of ligands by exponential enrichment (SELEX) technique and other random library technologys (such as phage display technology). Over the past 20 years the targets of SELEX have expanded to various metal ions, organic molecules, drugs, proteins and biomacromolecules, pathogenic bacteria, live cells and tissue slide. The SELEX screenings using live cells and tissue slide as targets have been preliminarily applied for studying targeted diagnosis and treatment of cancers. This paper reviews the expansion of the targets of SELEX technique. |
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| Keywords: | SELEX target random oligonucleotide library aptamer |
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