尿激酶型纤溶酶原活化物和基质金属蛋白酶类在腹主动脉瘤组织中的表达

目的探讨尿激酶型纤溶酶原活化物（u-PA）和明胶酶A（MMP-2）、明胶酶B（MMP-9）在腹主动脉瘤（AAA）组织中的表达及产生的源泉.方法用u-PA和MMP-2、MMP-9单克隆抗体以免疫组织化学方法在30例AAA组织和30例正常腹主动脉组织切片上检测u-PA和MMP-2、MMMP-9抗原（蛋白）;用u-PA的寡核苷酸探针和MMP-2、MMP-9的cDNA探针以原位杂交方法,在上述AAA组织和正常腹主动脉组织的冰冻切片上探测u-PA和MMP-2、MMP-9的mRNA.结果u-PA和MMP-9主要在AAA组织中层和外膜巨噬细胞表达,在正常腹主动脉组织中无表达;MMP-2在AAA组织中主要由中层平滑肌细胞表达,在正常腹主动脉组织中无表达.结论由巨噬细胞产生的u-PA直接激活原MMP-2和原MMP-9,在AAA形成和扩张中起着关键性作用.

Expression of urokiase-type plasminogen activator and matrix metalloproteinases in human tissue of abdominal aortic aneurysm

OBJECTIVE: To explore the expression of urokinase-type plasminogen activator (u-PA) and matrix metalloproteinases in human abdominal aortic aneurysm (AAA) tissue. METHODS: Immunohistochemistry and in situ hybridization were employed to detect the protein and mRNA expression of u-PA, gelatinase A (MMP-2) and gelatinase B (MMP-9) in the tissue sections of 30 AAA specimens and 30 normal human abdominal aorta specimens. RESULTS: The expressions of u-PA and MMP-9 were mainly found in the macrophages in AAA tissue, but not in normal human abdominal aorta tissues. The expression of MMP-2 was found mainly in the smooth muscle cells in AAA tissues, but not in normal human abdominal aorta tissues. CONCLUSION: u-PA produced by macrophages may immediately activate proMMP-2 and proMMP-9, which play a pivotal role in the formation and development of AAA.