| 酸碱胁迫对人参不定根中皂苷积累的影响及皂苷的组织化学定位研究 |
| |
| 引用本文： | 李铁军,廉美兰,邵春绘,于丹,朴炫春. 酸碱胁迫对人参不定根中皂苷积累的影响及皂苷的组织化学定位研究[J]. 中国中药杂志, 2013, 38(24): 4277-4280 |
| |
| 作者姓名： | 李铁军 廉美兰 邵春绘 于丹 朴炫春 |
| |
| 作者单位： | 延边大学 长白山生物资源与功能分子教育部重点实验室, 吉林 延吉 133002;延边大学 长白山生物资源与功能分子教育部重点实验室, 吉林 延吉 133002;延边大学 长白山生物资源与功能分子教育部重点实验室, 吉林 延吉 133002;延边大学 长白山生物资源与功能分子教育部重点实验室, 吉林 延吉 133002;延边大学 长白山生物资源与功能分子教育部重点实验室, 吉林 延吉 133002 |
| |
| 基金项目： | 国家自然科学基金项目（81160497） |
| |
| 摘 要： | 为了研究酸碱胁迫对人参不定根中皂苷含量的影响，不定根在反应器中培养30 d后进行酸碱处理的结果，酸碱处理可提高不定根中皂苷含量。用酸和碱处理后皂苷最高含量出现的时期不同，酸处理后的第5天人参皂苷质量分数达最高（14.0 mg·g-1 DW），而碱处理在第7天出现皂苷质量分数的最高值（14.1 mg·g-1 DW）。对不定根中皂苷进行组织化学定位的研究表明，不定根组织中皂苷的颜色呈现出浅红到深红的颜色变化，皂苷主要积累于中柱细胞。
|
| 关 键 词： | 人参不定根 酸碱胁迫 解剖结构 组织化学定位 |
| 收稿时间： | 2013-06-20 |
Effects of acid and alkali stress on ginsenoside content and histochemical localization of ginsenoside in adventitious root of Panax ginseng |
| |
| LI Tie-jun,LIAN Mei-lan,SHAO Chun-hui,YU Dan and PIAO Xuan-chun. Effects of acid and alkali stress on ginsenoside content and histochemical localization of ginsenoside in adventitious root of Panax ginseng[J]. China Journal of Chinese Materia Medica, 2013, 38(24): 4277-4280 |
| |
| Authors: | LI Tie-jun LIAN Mei-lan SHAO Chun-hui YU Dan PIAO Xuan-chun |
| |
| Affiliation: | Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University, Yanji 133002, China;Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University, Yanji 133002, China;Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University, Yanji 133002, China;Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University, Yanji 133002, China;Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular Yanbian University, Yanji 133002, China |
| |
| Abstract: | To investigate the effect of acid and alkali stress on ginsenoside content of Panax ginseng, adventitious roots culture in bioreactors were incubated for 30 d and pH value was adjusted. Ginsenoside content increased by reducing or raising the pH in culture medium, the muxium ginsenoside content was determined on the 5th days after acid treatment and on the 7th days after alkali treatment. The result of histochemical localization of ginsenoside revealed that the red color from light to dark were found in the adventitious root tissue, and ginsenoside mainly located in the pericycle cells where appeared the dark red color. |
| |
| Keywords: | adventitious root acid and alkali stress anatomical structure histochemical localization |
|
|
