人原发性食管癌组织中多种抑癌基因的研究

应用ＰＣＲ扩增与直接测序技术，分析１０例食管癌和癌旁组织标本中多个抑癌基因ｐ５３、Ｒｂ、ＡＰＣ和ＭＣＣ的突变与丢失。结果：１０例中６例有ｐ５３基因突变；５例有Ｒｂ基因改变；３例有ＡＰＣ改变；３例有ＭＣＣ基因改变；８例食管癌标本中至少有一种抑癌基因有改变；６例至少有两种或多种抑癌基因改变，１例癌旁组织中也有两个抑癌基因改变。结果表明，食管癌癌变与多个抑癌基因变化的累积有关。

The multiple tumor suppressor genes in humanesophageal cancer

he mutation and deletion of the multiple tumorsuppressor genes, including p53, Rb, APC and MCCin the same tissue of humanesophageal cancer (EC)and adjacent non-tumor were analysed by PCR am-plification and direct sequencing. In 10 cases of EC 6were found mutationsof p53 gene , 5 were found ab-normiality of Rb gene , 3 were found mutation ofAPC gene, 3 were found mutation of MCC gene, 8were found atleration abnormality of tumorsuppressor genes Rb, p53, APC and MCC, 6 werefound two or more abnormality of the tumorsuppressor genes. The results indicated that the altera-tions in multiple of tumor suppressor genes were re-lated to carcinogenesis of human esophageal cancer.