颌骨造釉细胞瘤中骨形成蛋白基因表达的原位杂交及免疫组化研究

利用骨形成蛋白（ＢＭＰ）单克隆抗体、ＢＭＰ多克隆抗体及地高辛甙元标记的ＢＭＰ１、ＢＭＰ２Ａ、ＢＭＰ３的ｃＤＮＡ探针，分别进行了４０例颌骨造釉细胞瘤中ＢＭＰ基因表达的原位杂交和免疫组化研究。结果发现，３种ＢＭＰ某因在造釉细胞瘤中均有过量表达，尤以ＢＭＰ２Ａ最为显著；与原位杂交结果相反，免疫组化显示，４０例造釉细胞瘤均为阴性。因此，ＢＭＰ基因的过量表达可能与造釉细胞瘤的发生有关，造釉细胞瘤中ＢＭＰ的产量可能较少，或其结构发生了改变。

In situ hvbridization and immunohistochemical detec-tion of bone morphogenetic protein gcnes inameloblastomas

one morphogenetic protein (BMP) is related tothe formation of bone and dentin.Imrnunohis-tochemical study showed that ameloblasts,odonto-blasts and dental papilla cells contained much BMP.Itis indicated BMP might be asaseiated with the epothe-lial-mesenchymal interaction of tooth germs.In thisstudy,the expression and distribution of BMP in 40patients with ameloblastomas were observed by usingin situ hybridization and immunohistochemistry. Theresults showed that all three BMP genes were overex-pressed in the epithelial component of ameloblastomas,however, no positive reaction was found in immuno-histochemistry.It is indicated that overexpression ofBMP genes might be related to the development ofameloblastomas.