班氏丝虫宿主循环抗原的检测

应用兔抗牛丝虫抗体ＥＬＩＳＡ双抗体夹心法和Ｄｏｔ－ＥＬＩＳＡ法检测安徽流行区人体班氏丝虫循环抗原。微丝蚴血症者血清总阳性率９６％（２４／２５），尿液总阳性率为６０％（１５／２５），４份乳汁阳性率均为１００％。１５份尿液阳性和４份乳汁阳性者与血清阳性结果的符合率分别为１００％（１５／１５）和７５％（３／４）。检测江苏非流行区正常人血清、尿液、乳汁共４３份，全部阴性。１１份肠道线虫感染者尿液全为阴性，血清Ｄｏｔ－ＥＬＩＳＡ阴性。１０份肺吸虫感染者血清Ｄｏｔ－ＥＬＩＳＡ阴性。结果表明，班氏丝虫循环抗原不仅存在于血清而且存在于尿液和乳汁中。

Parasitic circulating antigens in hosts with Filariabancrofti

he ELISA and Dot-ELISA with rabbitanti-Setaria labiat-papillosa antibody were applied todetermine circulating antigens of Filaria bancrofti.The total positive rate was 96% (24 / 25) in the sera ofpatients with microfilaremia from endemic areas,Anhui province. The total positive rate of urines was60% (15 / 25) in the same patients. The positive ratesof the milks were both 100% (4/4) in the same pa-tients. The conformity rate was 100% and 75%respectively for positive sera and unnes (15 cases).milks (4). Negative reactions were observed in the sera,urines and milks of all the 43 healthy people fromnon-endemic areas in Jiangsu province. Negative re-actions were observed in the unnes of all the 11non-filaria cases harbouring intestinal nematodas.The positive rate detected with ELISA was 36%(4 /11) in the patient sera that were negative whenthey were detected by Dot-ELISA. Negative reactionswere detected with Dot-ELISA in the sera of all the10 non-filaria patients infected with Paragonimuswestermani. The results showed that circulatingantigens of Filaria bancrofti exist not only in the se-rum but also in the urine and the milk.