| 外源性基因在大鼠血管平滑肌细胞中的表达 |
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| 引用本文： | 姚阿卿 孙双丹. 外源性基因在大鼠血管平滑肌细胞中的表达[J]. 中华医学杂志, 1994, 74(4): 224-227 |
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| 作者姓名： | 姚阿卿 孙双丹 |
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| 作者单位： | 北京医科大学心血管基础研究所，河北医学院，军事医学科学院，上海肿瘤研究所 |
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| 摘 要： | 分别构建了带有标记基因ＬａｃＺ和人的全长尿激酶原（ＰｒｏＵＫ）或纤溶酶原激活物（ｔＰＡ）基因的逆转录病毒载体，通过磷酸钙共沉淀法或假病毒感染法，转染血管平滑肌细胞，经Ｇ４ｌ８筛选得到抗性集落。分析证明，外源性基因可以整合到细胞染色体上，并可表达出相应的具有生物活性的蛋白质。提示血管平滑肌细胞可以作为心血管疾病基因治疗的靶细胞。
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| 关 键 词： | 基因疗法，基因表达调节，肌，平滑，血管 |
Foreign genes exprcssion in rat vascular smooth muselecells |
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| Yao Aqing,Sun Shangdan, Zhu Xiaojun,et al.. Foreign genes exprcssion in rat vascular smooth muselecells[J]. Zhonghua yi xue za zhi, 1994, 74(4): 224-227 |
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| Authors: | Yao Aqing Sun Shangdan Zhu Xiaojun et al. |
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| Affiliation: | Yao Aqing,Sun Shangdan, Zhu Xiaojun, et al. |
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| Abstract: | hree retroviral vectors containing report geneLacZ or the whole length cDNA of human Pro-UKand tPA, pN2-LacZ, pN2-CMV-ProUK andpN2-CMV-tPA were constructed. The rat vascularsmooth muscle cells (VSMCs) were transfected withthe plasmids by calcium phosphate coprecipitation orpseudovirus infection. The transfected cells were se-lected by G418. Southern blot analysis showed thatthe foreign genes were present in the genome of thetransfected VSMC, and the expression products ofthese genes could be detected in the transfectedVSMCs. These results suggested that the VSMC couldbe a targeting cell for gene therapy on cardiovasculardiseases. |
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| Keywords: | Gene therapy Gene expres-sion regulation Muscle smooth vascular |
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