RAB5A正义表达载体对两种人肺腺癌细胞系粘附基底膜成份能力的影响

目的　RAB5A基因过表达与人肺腺癌细胞系Anip 973的高转移性相关 ,进一步确认该基因在人低转移肺腺癌细胞系SPC a1和人低分化肺腺癌细胞系GLC 82中的作用。方法　采用癌细胞粘附基底膜成分的测定方法 ,分析RAB5A正义真核表达载体转染后的SPC a1、GLC 82两种肺腺癌细胞系的侵袭能力的改变。结果　RAB5A正义表达载体PcDNA3 .1 RAB5A转染的SPC a1较未转染细胞与基底膜成分粘附能力明显增强 ,t检验P <0 0 1;GLC 82细胞的侵袭能力作用不明显。结论　RAB5A在侵袭转移表型形成中发挥一定的作用

Study on the adhersion capability to ingredient of fundu s membrane in two human lungadenocarcinoma cells with RAB5A eukaryotic expres sion plasmid

Objective To prove over expression of RAB5A gene to be associated with neoplasia metastasis,to identify its function in low metastasis potential human lung adenocarcinoma cells SPC a 1 and low differentiation human lung adenocarcinoma cells GLC 82 more.Methods We use the method of adhesion capability of tumor cell in transfected and nontransfected cells respectively.Results After SPC a 1 cells were transfected by pcDNA3.1 RAB5A plasmid,its adhesion capability was significantly increased ( P <0.01);while GLC 82 cells was not so evident.Conclusion Our research in vitro indicate that over expression of RAB5A genes play a definite role in tumor invasion and metastatic phenotype formation of human lung adenocarcinoma cells.