Wnt信号通路调节因子在肝门部胆管癌组织中的表达

目的研究肝门部胆管癌组织中Wnt经典通路调节因子Frizzled、LRP5/6、APC、Axin的表达，结合既往的研究明确Wnt经典通路的整体调节。 方法以1998年4月至2007年5月病理确诊为肝门部胆管癌的129例肿瘤标本作为胆管癌组，使用同期的45例先天性胆总管囊肿标本作为对照（囊肿组），通过组织芯片技术检测所有标本的Wnt经典通路中主要调节因子Frizzled、LRP5/6、APC、Axin的表达。 结果囊肿组中Frizzled阳性率高于胆管癌组（91.1% vs 78.3%，H=9.29，P<0.05）；胆管癌组的LRP5/6及APC阳性率分别为91.5%、84.5%，囊肿组的LRP5/6及APC阳性率分别为95.6%、77.8%，两组差异均无统计学意义（H=0.622、1.800，P=0.733、0.400）；而Axin在胆管癌组的表达阳性率显著高于囊肿组（91.4% vs 88.9%，H=6.23，P<0.05）。 结论以组织芯片技术为手段，本研究反映出肝门部胆管癌Wnt通路中Frizzled及Axin两种蛋白较囊肿组织体现显著差异，有望成为诊断及治疗的靶点。

Expression of regulatory factors in Wnt signaling pathway in tissue specimens of patients with hilar cholangiocarcinoma

ObjectiveTo investigate the role of regulatory factors in hilar cholangiocarcinoma according to the expression of Wnt canonical signaling pathway in tissue specimens. MethodsThe archived pathology specimens of one hundred and twenty-nine patients with hilar cholangiocarcinoma were collected to construct the tissue microarray between April 1998 and May 2007, and 45 cases of congenital choledochal cyst were considered as control group. The expression of Frizzled, LRP5/6, APC, Axin, as regulatory factors and target genes, were detected by using tissue microarray and were compared between different diseases. ResultsThe expression of Frizzled was higher in congenital choledochal cyst, compared to hilar cholangiocarcinoma (91.1% vs 78.3%, H=9.29, P<0.05). There was no significant differences of LRP5/6 and APC between the two groups (91.5% vs 95.6%, 84.5% vs 77.8%, H=0.622, 1.800, P=0.733, 0.400). The positive expression of Axin was significantly higher in hilar cholangiocarcinoma compared with congenital choledochal cyst (91.4% vs 88.9%, H=6.23, P<0.05). ConclusionsIt shows that the expression of Frizzled and Axin in hilar cholangiocarcinoma is different from that in congenital choledochal cyst by tissue microarray. The two Wnt signaling pathway-related factors may be potentially valuable target for diagnosis or treatment.