生物钟基因CLOCK蛋白对甲状腺乳头状癌的影响

目的探讨生物钟基因CLOCK在甲状腺乳头状癌组织中的蛋白表达，并分析其与甲状腺癌病理特征的关系。 方法收集2012年3月到2015年3月在中国人民解放军第九七医院手术切除的甲状腺乳头状癌标本74份，采用免疫组化方法检测CLOCK蛋白在甲状腺乳头状癌及配对癌旁组织中的表达水平，分析其表达与临床病理特征的关系。 结果甲状腺乳头状癌组织与其配对的癌旁正常组织中CLOCK蛋白阳性表达分别为68.9%（51/74）、20.3%（15/74），两类组织中阳性率的差异有统计学意义（χ²=35.44，P=0.000）。CLOCK蛋白表达情况与甲状腺乳头状癌淋巴结转移情况、肿瘤直径及TNM分期有关（P <0.05）。 结论CLOCK蛋白可能与甲状腺乳头状癌的发生、肿瘤的侵袭和转移等关系密切，可为甲状腺癌的早期诊断提供依据。

Significance of circadian genes clock protein in papillary thyroid carcinoma tissues

ObjectiveTo investigate the protein expression level of CLOCK gene in papillary thyroid carcinoma (PTC) tissues and the relationship between the expression level and pathological features of patients with PTC. MethodsPTC patients undergoing radical surgery at Affliated PLA No. 97 Hospital from March 2012 to March 2015 were collected. Finally, seventy four cases were enrolled in this study. The expression level of CLOCK protein was analyzed by immunohistochemistry in PTC tissues and the paired adjacent normal thyroid tissues. The relationship between the expression level of CLOCK protein and clinicopathological features were also analyzed. ResultsThe positive expression rates of CLOCK protein in PTC tissues and paired adjacent normal tissues were 68.9% (51/74) and 20.3% (15/74), respectively, with significant difference (χ²=35.44, P=0.000). The expression level of CLCOK protein in thyroid cancer was related to lymph node metastasis, diameter of tumor and TNM stage. ConclusionThe CLOCK protein plays an important role in tumorigenesis, tumor invasion and metastasis.