冷保存大鼠部分肝移植模型的建立

目的　建立稳定的大鼠部分肝移植模型 ,研究冷保存对部分肝移植后肝再生的影响。方法　采用雄性SD大鼠 ,双袖套法行部分肝移植 ,观察不同冷保存时间受体生存状况以及各组手术情况。结果　各组移植肝平均体积为 62 % -64 % ,无肝期为 14 -15min。长时间冷保存后大鼠 7d存活率明显下降 ,生存分析显示各组间差异有显著性。 8h冷保存后大鼠 7d存活率为 40 % ,与临床辟裂式肝移植 1年生存率相似。结论　 8h保存大鼠部分肝移植模型是研究冷保存对部分肝移植肝再生影响的较好模型

A model of partial liver transplantation with extended cold preservation in rats

Objective To develop a model of partial liver transplantation (PLT) in rats to study the impact of cold preservation on liver regeneration after PLT. Methods The PLT was performed in SD rat using the two-cuff technique. Survival condition and operation parametes were observed. Results The average volume of grafts was 62% to 64% of the recipient liver; and the anhepatic phase was 14 to 15 min in all groups. After extended cold preservation, the 7-day survival rate of recipients was decreased significantly. The 7-day survival rate of recipients with preserved for 8 h was 40%. Conclusions The PLT model in rats with grafts undergoing 8-hour cold preservation, which is comparable with the split liver transplantation in humans, provides suitable model for studying the effects of cold preservation on liver regeneration after PLT.